Determination of protein in preparations of microsomes.
Determination of protein in preparations of microsomes.
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微粒体制剂中蛋白质的测定。
DOI：
10.1016/0003-2697(75)90458-3
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发表时间：
1975
影响因子：
2.9
通讯作者：
P. Albro
中科院分区：
文献类型：
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作者：
P. Albro
比较了基于苯酚、缩二脲和苦基磺酸盐反应的九种蛋白质测定程序，以确定它们是否适用于测定微粒体制剂中的蛋白质。除了显示光散射干扰的“大缩二脲”和 Lowry 等人的常规检测之外，所有方法都给出了可比较的结果。 (1) 以及 Schacterle 和 Pollack (14) 给出了钙沉淀微粒体的低值。讨论了各种测定在简单性、干扰和灵敏度方面的优点和缺点。特别推荐三种测定方法，即“不溶性缩二脲”(17)、“非水性缩二脲”(18) 以及 Schacterle 和 Pollack (14) 描述的测定法的修改（这是对 Lowry 等人 (1) 测定法的修改）。
Nine protein assay procedures based on the phenol, biuret, and picryl sulfonate reactions were compared as to their suitability for the determination of protein in microsomal preparations. All of the methods gave comparable results except the “macrobiuret,” which showed light-scattering interference, and the routine assays of Lowry et al. (1) and Schacterle and Pollack (14) which gave low values with calcium-precipitated microsomes. The advantages and disadvantages of the various assays relative to simplicity, interferences, and sensitivity are discussed. Three of the assay methods, the “insoluble biuret” (17) “nonaqueous biruet” (18), and a modification of the assay described by Schacterle and Pollack (14) (which is a modification of the assay of Lowry et al. (1), are particularly recommended.