The Interaction of a Histidine-Rich Protein Hpn with the Membrane Mimics: Implications for Pathologic Roles of Hpn in Helicobacter pylori
The Interaction of a Histidine-Rich Protein Hpn with the Membrane Mimics: Implications for Pathologic Roles of Hpn in Helicobacter pylori
复制标题
富含组氨酸的蛋白 Hpn 与膜模拟物的相互作用：对 Hpn 在幽门螺杆菌中病理作用的影响
DOI：
10.1111/hel.12109
复制
发表时间：
2014
期刊：
影响因子：
4.4
通讯作者：
Ge Ruiguang
中科院分区：
文献类型：
--
作者：
Zhou Qinglu;Qi Shuang;Sun Xuesong;Ge Ruiguang
背景Hpn 是幽门螺杆菌中一种富含组氨酸的小蛋白。该蛋白质已被证明在镍储存和解毒中发挥作用，并对胃上皮细胞表现出细胞毒性。 Hpn可以分泌到细菌外，形成类淀粉样结构。目的研究Hpn与膜模拟物之间的相互作用，以进一步了解该细菌的病理作用。方法采用CD二级结构测定、荧光光谱法钙黄绿素释放测定、Laurdan和Prodan广义偏振测定等多种生化和生物物理方法来表征Hpn与膜模拟物之间的相互作用。模拟物诱导 Hpn 中 α 螺旋的形成。这种相互作用破坏了膜模拟物的完整性，并导致内部钙黄绿素探针的释放。涉及 Laurdan 和 Prodan 荧光的实验表明，增加总蛋白/脂质比会导致靠近模拟线粒体内膜的脂质双层的水/脂质界面的有序度较低且更加水合的脂质膜结构。结论目前的数据表明，Hpn 可能通过膜相互作用参与幽门螺杆菌的病理作用。
BackgroundHpn is a small histidine‐rich protein in Helicobacter pylori. This protein has been shown to play roles in nickel storage and detoxification and to exhibit cytotoxicity to gastric epithelial cells. Hpn can be secreted outside of the bacterium and forms amyloid‐like structures.ObjectiveTo study the interactions between Hpn and membrane mimics, which may further our understanding of the pathologic roles of this bacterium.MethodsVarious biochemical and biophysical methods, such as secondary structure determination be CD, calcein release assay with fluorescence spectrometry, and Laurdan and Prodan generalized polarization determination have been used to characterize the interaction between Hpn and membrane mimics.ResultsMembrane mimics induced the formation of α‐helix in Hpn. The interaction disrupts the integrity of the membrane mimics and leads to the release of inner calcein probe. The experiments involving the Laurdan and Prodan fluorescence indicated that increasing the total protein/lipid ratio leads to a less ordered and more hydrated lipid membrane structure close to the water/lipid interface of lipid bilayers modeling the mitochondrial inner membrane.ConclusionThe present data indicated that Hpn may take part in the pathological roles of Helicobacter pylori through membrane interactions.