Functional and anatomical localization of mu opioid receptors in the striatum, amygdala, and extended amygdala of the nonhuman primate
Functional and anatomical localization of mu opioid receptors in the striatum, amygdala, and extended amygdala of the nonhuman primate
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DOI：
10.1002/cne.1154
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发表时间：
2001-05-14
影响因子：
2.5
通讯作者：
Porrino, LJ
中科院分区：
文献类型：
--
作者：
Daunais, JB;Letchworth, SR;Porrino, LJ
在食蟹猴的纹状体、杏仁核和扩展杏仁核中检查μ阿片受体的次区域分布和相应的G蛋白活化。使用放射自显影与[H-3]DAMGO，一种选择性mu配体的mu结合位点的地形定义。在相邻切片中，通过DAMGO刺激的鸟苷酰5 '（γ-[S-35]硫代）三磷酸（[S-35]GTP γ S）结合鉴定受体活化G蛋白的分布。在纹状体内，[3 H]DAMGO结合位点的分布的特点是由一个独特的背腹侧梯度与更高浓度的结合位点在更吻侧水平的纹状体。[H-3]DAMGO结合进一步通过尾状核内的斑块样聚集体的存在以及非常密集的受体结合位点的较小区域来区分，所述受体结合位点先前在人纹状体中被鉴定为神经化学上独特的核和壳核结构域（NUDAP）。杏仁核中含有最高浓度的[H-3]DAMGO结合位点，在基底核、副基底核、杏仁核和内侧核中观察到明显的结合水平。在纹状体和杏仁核中，DAMGO刺激的G蛋白激活的分布在很大程度上与mu结合位点的分布相对应。然而，杏仁核的中央核和内侧核是明显的例外。虽然杏仁核中央核中[H-3]DAMGO结合位点的浓度非常低，但与含有更高浓度[H-3]DAMGO结合位点的杏仁核其他部分相比，该核中DAMGO刺激的G蛋白活化浓度（用[S-35] GTP γ S结合测定）相对较高。内侧核的情况正好相反，高浓度的结合位点与较低水平的DAMGO刺激的G蛋白激活相关。最后，[H-3]DAMGO和[S-35]GTP γ S在杏仁核内结合，特别是内侧核，与无名质和终纹床核形成连续体，支持灵长类动物杏仁核延长的概念。(C)2001 Wiley-Liss，Inc.
The subregional distribution of mu opioid receptors and corresponding G-protein activation were examined in the striatum, amygdala, and extended amygdala of cynomolgus monkeys. The topography of mu binding sites was defined using autoradiography with [H-3]DAMGO, a selective mu ligand. In adjacent sections, the distribution of receptor-activated G proteins was identified with DAMGO-stimulated guanylyl 5 '(gamma-[S-35]thio)triphosphate( [S-35]GTP gammaS) binding. Within the striatum, the distribution of [3H]DAMGO binding sites was characterized by a distinct dorsalventral gradient with a higher concentration of binding sites at more rostral levels of the striatum. [H-3]DAMGO binding was further distinguished by the presence of patch-like aggregations within the caudate, as well as smaller areas of very dense receptor binding sites, previously identified in human striatum as neurochemically unique domains of the accumbens and putamen (NUDAPs). The amygdala contained the highest concentration of [H-3]DAMGO binding sites measured in this study, with the densest levels of binding noted within the basal, accessory basal, paralaminar, and medial nuclei. In the striatum and amygdala, the distribution of DAMGO-stimulated G-protein activation largely corresponded with the distribution of mu binding sites. The central and medial nuclei of the amygdala, however, were notable exceptions. Whereas the concentration of [H-3]DAMGO binding sites in the central nucleus of the amygdala was very low, the concentration of DAMGO-stimulated G-protein activation in this nucleus, as measured with [S-35] GTP gammaS binding, was relatively high compared to other portions of the amygdala containing much higher concentrations of [H-3]DAMGO binding sites. The converse was true in the medial nucleus, where high concentrations of binding sites were associated with lower levels of DAMGO-stimulated G-protein activation. Finally, [H-3]DAMGO and [S-35]GTP gammaS binding within the amygdala, particularly the medial nucleus, formed a continuum with the substantia innominata and bed nucleus of the stria terminalis, supporting the concept of the extended amygdala in primates. (C) 2001 Wiley-Liss, Inc.