Glutathione (GSH)-decorated magnetic nanoparticles for binding glutathione-S-transferase (GST) fusion protein and manipulating live cells
Glutathione (GSH)-decorated magnetic nanoparticles for binding glutathione-S-transferase (GST) fusion protein and manipulating live cells
复制标题
DOI：
10.1039/c1sc00030f
复制
发表时间：
2011-01-01
期刊：
影响因子：
8.4
通讯作者：
Xu, Bing
中科院分区：
文献类型：
--
作者：
Pan, Yue;Long, Marcus J. C.;Xu, Bing
通过多巴胺锚定表面修饰谷胱甘肽（GSH）的氧化铁磁性纳米颗粒（MNP）以高亲和力和特异性结合人α - 1-谷胱甘肽s -转移酶（GST），并能够从细胞裂解物中分离GST融合蛋白。纯化的GST和感兴趣的蛋白（POI）都保留了它们固有的特性。MNP和GST融合蛋白的结合也使磁性操纵细胞成为可能。
Iron oxide-based magnetic nanoparticles (MNP) surface-decorated with glutathione (GSH) via a dopamine anchor bind to human alpha 1-glutathione S-transferase (GST) with high affinity and specificity and are able to separate GST fusion proteins from cell lysates. Both the purified GST and the protein of interest (POI) preserve their innate properties. The conjugate of MNP and the GST fusion protein also enables magnetic manipulation of cells.