Functional synapse formation between rat olfactory receptor neurons and olfactory bulb neurons in vitro
Functional synapse formation between rat olfactory receptor neurons and olfactory bulb neurons in vitro
复制标题
体外大鼠嗅觉受体神经元与嗅球神经元之间的功能性突触形成
DOI：
10.1016/s0304-3940(00)01028-4
复制
发表时间：
2000
影响因子：
2.5
通讯作者：
M. Kashiwayanagi
中科院分区：
文献类型：
--
作者：
K. Kanaki;Kimihiko Sato;M. Kashiwayanagi
为探讨嗅受体神经元与嗅球神经元间突触形成的机制，采用改良的薄片培养方法，将嗅上皮块与嗅球切片共培养。培养4 d后，观察到嗅上皮块的神经元特异性纤维延伸到嗅球切片。培养约20天后，在电压钳条件下，将诱导嗅觉受体神经元兴奋反应的福斯克林应用于上皮诱导嗅球神经元向内电流反应，表明在体外条件下，嗅觉受体神经元和嗅球神经元之间的功能突触已经建立。
To explore the mechanism of the synapse formation between olfactory receptor neurons and neurons in the olfactory bulb, blocks of olfactory epithelium and slices of olfactory bulb were cocultured by a modified slice culture method. After 4 days in culture, neuron specific fibers from the olfactory epithelium block extending to the olfactory bulb slice were observed. After ∼20 days in culture, application of forskolin, which induces excitatory responses in olfactory receptor neurons, to the epithelium induced inward current responses in olfactory bulb neurons under the voltage clamp conditions, indicating that functional synapses between olfactory receptor neurons and olfactory bulb neurons had been established in vitro conditions.