Monocyte differentiation and macrophage priming are regulated differentially by pentraxins and their ligands.
Monocyte differentiation and macrophage priming are regulated differentially by pentraxins and their ligands.
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DOI：
10.1186/s12865-017-0214-z
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发表时间：
2017-06-15
期刊：
影响因子：
3
通讯作者：
Gomer RH
中科院分区：
文献类型：
--
作者：
Pilling D;Galvis-Carvajal E;Karhadkar TR;Cox N;Gomer RH
循环骨髓来源的单核细胞可以离开血液，进入组织，并分化为M1炎性、M2 a重塑/纤维化或M2 c/Mreg分辨/免疫调节巨噬细胞。宏程序也可以从上述类型之一转换为另一种类型。正五聚素是分泌的蛋白质，其在感染、炎症和组织损伤期间与微生物病原体和细胞碎片结合并促进微生物病原体和细胞碎片的有效清除。正五聚蛋白C反应蛋白（CRP）、血清淀粉样蛋白P（SAP）和正五聚蛋白-3（PTX 3）也可结合多种内源性配体。由于单核细胞和巨噬细胞在感染、炎症和组织损伤期间暴露于不同浓度的五聚素及其配体，我们评估了五聚素及其配体对这些细胞的影响。我们发现，许多极化标记物不能区分pentraxins及其配体对巨噬细胞的影响。然而，正五聚素，它们的配体，和细胞因子差异调节血红蛋白-触珠蛋白复合物受体CD 163，唾液酸结合凝集素CD 169，和巨噬细胞甘露糖受体CD 206的表达。CRP是一种通常被认为是促炎性的五聚环蛋白，它增加抗炎细胞因子IL-10的细胞外积累，并且这种作用被GM-CSF、甘露糖结合凝集素和因子H减弱。这些结果表明，存在的pentraxins和它们的配体调节血液和组织中的巨噬细胞分化，CRP可能是一个有效的诱导剂的抗炎细胞因子IL-10。本文的在线版本（doi：10.1186/s12865-017-0214-z）包含补充材料，可供授权用户使用。
Circulating bone marrow-derived monocytes can leave the blood, enter a tissue, and differentiate into M1 inflammatory, M2a remodeling/fibrotic, or M2c/Mreg resolving/immune-regulatory macrophages. Macrophages can also convert from one of the above types to another. Pentraxins are secreted proteins that bind to, and promote efficient clearance of, microbial pathogens and cellular debris during infection, inflammation, and tissue damage. The pentraxins C-reactive protein (CRP), serum amyloid P (SAP), and pentraxin-3 (PTX3) can also bind a variety of endogenous ligands. As monocytes and macrophages are exposed to differing concentrations of pentraxins and their ligands during infection, inflammation, and tissue damage, we assessed what effect pentraxins and their ligands have on these cells. We found that many polarization markers do not discriminate between the effects of pentraxins and their ligands on macrophages. However, pentraxins, their ligands, and cytokines differentially regulate the expression of the hemoglobin-haptoglobin complex receptor CD163, the sialic acid-binding lectin CD169, and the macrophage mannose receptor CD206. CRP, a pentraxin generally thought of as being pro-inflammatory, increases the extracellular accumulation of the anti-inflammatory cytokine IL-10, and this effect is attenuated by GM-CSF, mannose-binding lectin, and factor H. These results suggest that the presence of pentraxins and their ligands regulate macrophage differentiation in the blood and tissues, and that CRP may be a potent inducer of the anti-inflammatory cytokine IL-10. The online version of this article (doi:10.1186/s12865-017-0214-z) contains supplementary material, which is available to authorized users.