Mineralization promotion and protection effect of carboxymethyl chitosan biomodification in biomimetic mineralization
Mineralization promotion and protection effect of carboxymethyl chitosan biomodification in biomimetic mineralization
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DOI：
10.1016/j.ijbiomac.2023.123720
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发表时间：
2023-02-20
影响因子：
8.2
通讯作者：
Zhang，Linglin
中科院分区：
文献类型：
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作者：
Li，Zhongcheng;Zeng，Yuhao;Zhang，Linglin
仿生矿化强调通过生物启发的策略逆转龋齿的过程，其中促进矿化和保护胶原蛋白同等重要。本研究以羧甲基壳聚糖（CMC）作为糖胺聚糖的类似物，对胶原进行仿生改性，评价CMC对胶原的矿化促进作用和保护作用。同时在二维单层重建胶原模型、三维重建胶原模型和脱矿牙本质模型上进行实验。在三种模型中，利用生物相容的1-乙基-3-（3-二甲氨基丙基）碳二亚胺盐酸盐和N-羟基磺基琥珀酰亚胺钠盐将CMC成功地交联到胶原上以实现生物改性。结果表明，CMC生物改性提高了胶原蛋白的亲水性、钙吸收能力和耐热降解性能。在脱矿牙本质模型中，CMC生物改性显著抑制内源性基质金属蛋白酶的活性。此外，CMC生物改性显着改善胶原的交联和纤维内矿化，特别是在二维单层重建胶原模型。本研究提供了一种综合考虑胶原保护的仿生矿化策略，丰富了壳聚糖基材料在口腔医学中的应用。
Biomimetic mineralization emphasizes reversing the process of dental caries through bio-inspired strategies, in which mineralization promotion and collagen protection are equally important. In this study, carboxymethyl chitosan (CMC) was deemed as an analog of glycosaminoglycan for biomimetic modification of collagen, both of the mineralization facilitation and collagen protection effect were evaluated. Experiments were carried out simultaneously on two-dimensional monolayer reconstituted collagen model, three-dimensional reconstituted collagen model and demineralized dentin model. In three models, CMC was successfully cross-linked onto collagen utilizing biocompatible 1-Ethyl-3(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxy sulfosuccinimide sodium salt to achieve biomodification. Results showed that CMC biomodification increased collagen's hydrophilicity, calcium absorption capacity and thermal degradation resistance. In demineralized dentin model, the activity of endogenous matrix metalloproteinases was significantly inhibited by CMC biomodification. Furthermore, CMC biomodification significantly improved cross-linking and intrafibrillar mineralization of collagen, especially in the two-dimensional monolayer reconstituted collagen model. This study provided a biomimetic mineralization strategy with comprehensive consideration of collagen protection, and enriched the application of chitosan-based materials in dentistry.