Proteomics characterization of exosome cargo.
Proteomics characterization of exosome cargo.
复制标题
DOI：
10.1016/j.ymeth.2015.03.018
复制
发表时间：
2015-10-01
期刊：
影响因子：
--
通讯作者：
Rose KL
中科院分区：
文献类型：
--
作者：
Schey KL;Luther JM;Rose KL
外泌体货物的表征具有重要意义，因为这种货物可以提供外泌体生物发生、靶向和细胞效应的线索，并且可以是用于疾病诊断、预后和对治疗的反应的生物标志物的来源。随着近年来蛋白质组学技术的进步，外泌体蛋白质的定性和定量表征成为可能。在这里，我们提供了一个简短的审查外泌体蛋白质组学的研究，并提供详细的协议，全球定性，全球定量和有针对性的定量分析外泌体蛋白。此外，我们提供了标准全局定量分析的示例应用，然后通过对来自人类患者的尿液外泌体样品进行靶向定量分析进行验证。每种方法的优点和局限性进行了讨论，以及外来体蛋白质组学分析的未来方向。
Characterization of exosomal cargo is of significant interest because this cargo can provide clues to exosome biogenesis, targeting, and cellular effects and may be a source of biomarkers for disease diagnosis, prognosis and response to treatment. With recent improvements in proteomics technologies, both qualitative and quantitative characterization of exosomal proteins is possible. Here we provide a brief review of exosome proteomics studies and provide detailed protocols for global qualitative, global quantitative, and targeted quantitative analysis of exosomal proteins. In addition, we provide an example application of a standard global quantitative analysis followed by validation via a targeted quantitative analysis of urine exosome samples from human patients. Advantages and limitations of each method are discussed as well as future directions for exosome proteomics analysis.