Enrichment culture of electroactive microorganisms with high magnetic susceptibility enhances the performance of microbial fuel cells
Enrichment culture of electroactive microorganisms with high magnetic susceptibility enhances the performance of microbial fuel cells
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高磁化率电活性微生物的富集培养提高微生物燃料电池的性能
DOI：
10.1016/j.bioelechem.2018.01.005
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发表时间：
2018-06-01
影响因子：
5
通讯作者：
Liu, Fanghua
中科院分区：
文献类型：
--
作者：
Chen, Lei;Zhang, Peng;Liu, Fanghua
许多电活性微生物（EAM）被称为异化铁还原菌（DIRB），其在呼吸过程中可以将铁作为电子受体来传递电子。以前的研究表明，磁化率（chi lf）可以作为DIRB活动的指标。然而，chi lf和EAM电活性之间的关系仍然不清楚。在这里，我们报告了沙河沉积物富集无定形铁后，富集培养物的chi lf急剧增加。与低chi lf培养物相比，高chi lf培养物接种的反应器启动更早，并且具有更高的峰值电压（0.228 V）、更高的功率密度（310.14 mW m（-2））和更高的库仑效率（10.16%）。细菌16 S rRNA基因扩增子测序和chi lf测量显示，沉积物中优势门变形菌的相对丰度随着chi lf值的增加而增加。循环伏安曲线和细菌rRNA基因的细菌末端限制性片段长度多态性（T-RFLP）的进一步分析表明，提高性能的MFC可能是由于一个高度电活性的微生物群落，162 bp的末端限制性片段（TRFs）的起源。我们的研究结果表明，一个潜在的相关性，在复杂的环境中快速筛选的存在下，表明一个有前途的路线。(C)2018 Elsevier B. V.版权所有。
Many electroactive microorganisms (EAMs) are known as dissimilatory iron-reducing bacteria (DIRB) which can access iron as an electron acceptor to transfer electrons during the process of respiration. Previous research has shown that magnetic susceptibility (chi lf) could be used as an indicator of DIRB activities. However, the relationship between chi lf and electroactivity of EAMs is still unclear. Here, we report that after sediments of the Sha River enriched with amorphous iron, the chi lf of enrichment cultures increased dramatically. The reactor inoculated with higher chi lf enrichment cultures started earlier and had a higher peak voltage (0.228 V), higher power density (310.14 mW m(-2)) and higher coulombic efficiency (10.16%) than lower chi lf cultures. Sequencing of bacterial 16S rRNA gene amplicons and chi lf measurement revealed that relative abundance of dominant phyla Proteobacteria increased with chi lf values in sediments. Further analysis of cyclic voltammetry curves and bacterial terminal-restriction fragment length polymorphism (T-RFLP) of bacterial rRNA genes indicated that the improved performance of the MFCs might be due to a highly electroactive microbial community that the 162 bp terminal restriction fragments (TRFs) originated from. Our results suggested a potential correlation between chi lf and presence of EAM indicating a promising route for fast screening in complex environments. (C) 2018 Elsevier B.V. All rights reserved.