Pecans Acutely Increase Plasma Postprandial Antioxidant Capacity and Catechins and Decrease LDL Oxidation in Humans
Pecans Acutely Increase Plasma Postprandial Antioxidant Capacity and Catechins and Decrease LDL Oxidation in Humans
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DOI：
10.3945/jn.110.121269
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发表时间：
2011-01-01
影响因子：
4.2
通讯作者：
Sabate, Joan
中科院分区：
文献类型：
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作者：
Hudthagosol, Chatrapa;Haddad, Ella Hasso;Sabate, Joan
山核桃坚果的生物活性成分，如γ-生育酚和黄烷-3-酚单体，在体外显示出抗氧化特性，但人体生物利用度尚不清楚。我们研究了血浆氧自由基吸收能力（ORAC）和生育酚，儿茶素，氧化低密度脂蛋白和丙二醛（MDA）的浓度在响应山核桃测试餐后的变化。16名健康男性和女性（23-44岁，BMI 22.7 +/- 3.4）被随机分配到3个试验餐序列，包括全山核桃、混合山核桃或等量常量营养素组成的等热量餐，但采用交叉设计，治疗之间有1周的洗脱期。在基线和摄入后24小时内间隔采集血液样本。在完整和混合的山核桃试验餐后，γ-生育酚的血浆浓度在8小时加倍（P < 0.001），亲水性和亲脂性ORAC在2小时分别增加12%和10%。食用全山核桃后，氧化LDL在2、3和8小时分别降低30、33和26（P < 0.05），和表没食子儿茶素-3-没食子酸酯浓度在1h（平均值+/- SEM; 95.1 +/- 30.6 nmol/L）和2 h（116.3 +/- 80.5 nmol/L）高于基线（0 h）和对照试验餐后1 h（P < 0.05）。仅当合并完整和混合山核桃数据时，餐后MDA：甘油三酯摩尔比在3、5和8 h分别降低37、36和40%（P < 0.05）。这些结果表明，山核桃的生物活性成分是可吸收的，有助于餐后抗氧化防御。J.营养141：56-62，2011.
Bioactive constituents of pecan nuts such as gamma-tocopherol and flavan-3-ol monomers show antioxidant properties in vitro, but bioavailability in humans is not known. We examined postprandial changes in plasma oxygen radical absorbance capacity (ORAC) and in concentrations of tocopherols, catechins, oxidized LDL, and malondialdehyde (MDA) in response to pecan test meals. Sixteen healthy men and women (23-44 y, BMI 22.7 +/- 3.4) were randomly assigned to 3 sequences of test meals composed of whole pecans, blended pecans, or an isocaloric meal of equivalent macronutrient composition but formulated of refined ingredients in a crossover design with a 1-wk washout period between treatments. Blood was sampled at baseline and at intervals up to 24 h postingestion. Following the whole and blended pecan test meals, plasma concentrations of gamma-tocopherols doubled at 8 h (P < 0.001) and hydrophilic- and lipophilic-ORAC increased 12 and 10% at 2 h, respectively. Post whole pecan consumption, oxidized LDL decreased 30, 33, and 26% at 2, 3, and 8 h, respectively (P < 0.05), and epigallocatechin-3-gallate concentrations at 1 h (mean +/- SEM; 95.1 +/- 30.6 nmol/L) and 2 h (116.3 +/- 80.5 nmol/L) were higher than at baseline (0 h) and after the control test meal at 1 h (P < 0.05). The postprandial molar ratio of MDA:triglycerides decreased by 37, 36, and 40% at 3, 5, and 8 h, respectively (P < 0.05), only when whole and blended pecan data were pooled. These results show that bioactive constituent of pecans are absorbable and contribute to postprandial antioxidant defenses. J. Nutr. 141: 56-62, 2011.