Dual-wavelength oblique back-illumination microscopy for the non-invasive imaging and quantification of blood in collection and storage bags
Dual-wavelength oblique back-illumination microscopy for the non-invasive imaging and quantification of blood in collection and storage bags
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DOI：
10.1364/boe.9.002743
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发表时间：
2018-06-01
影响因子：
3.4
通讯作者：
Robles, Francisco E.
中科院分区：
文献类型：
--
作者：
Ledwig, Patrick;Sghayyer, Moses;Robles, Francisco E.
目前还没有一种低成本方法可以在不破坏血袋并可能损坏样本的情况下定量评估血袋的内容物。为此，我们采用斜背照式显微镜 (OBM) 来快速、廉价且无创地筛查血袋的红细胞 (RBC) 形态和白细胞 (WBC) 计数。 OBM 最近被引入作为一种断层摄影技术，可基于相位梯度和透射产生高分辨率宽视场图像。在这里，我们修改了该技术以包括双波长照明，以促进细胞分类的光谱分析。此外，我们应用改进的 2D Hilbert 变换从相位梯度图像中恢复相位信息，以实现轻松的细胞分割。血细胞分为白细胞和红细胞，并使用自动算法根据形状、吸收光谱和相位分布进行计数。这项工作对于以下方面的无创评估具有重要意义：(1) 用于输血应用的储存袋中的细胞活力和 (2) 用于干细胞治疗应用的脐带血采集袋的适用性。 (C) 2018 年美国光学协会根据 OSA 开放获取出版协议条款
There is currently no low-cost method to quantitatively assess the contents of a blood bag without breaching the bag and potentially damaging the sample. Towards this end, we adapt oblique back-illumination microscopy (OBM) to rapidly, inexpensively, and non-invasively screen blood bags for red blood cell (RBC) morphology and white blood cell (WBC) count. OBM has been recently introduced as a tomographic technique that produces high-resolution wide-field images based on phase-gradient and transmission. Here we modify this technique to include illumination at dual wavelengths to facilitate spectral analysis for cell classification. Further, we apply a modified 2D Hilbert transform to recover the phase information from the phase-gradient images for facile cell segmentation. Blood cells are classified as WBCs and RBCs, and counted based on shape, absorption spectrum, and phase profile using an automated algorithm. This work has important implications for the non- invasive assessment of (1) cell viability in storage bags for transfusion applications and (2) suitability of a cord blood collection bag for stem cell therapy applications. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement