A Modified System for Analyzing Ionizing Radiation-Induced Chromosome Abnormalities
A Modified System for Analyzing Ionizing Radiation-Induced Chromosome Abnormalities
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DOI：
10.1667/rr2849.1
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发表时间：
2012-05-01
影响因子：
3.4
通讯作者：
Tashiro, Satoshi
中科院分区：
文献类型：
--
作者：
Shi, Lin;Fujioka, Kurumi;Tashiro, Satoshi
The analysis of dicentric chromosomes in human peripheral blood lymphocytes (PBLs) by Giemsa staining is the most established method for biological dosimetry. However, this method requires a well-trained person because of the difficulty in detecting aberrations rapidly and accurately. Here, we applied a fluorescence in situ hybridization (FISH) technique, using telomere and centromere peptide nucleic acid (PNA) probes, to solve the problem of biological dosimetry in radiation emergency medicine. A comparison by a well-trained observer found that FISH analysis of PBLs for the dose estimation was more accurate than the conventional Giemsa analysis, especially in samples irradiated at high doses. These results show that FISH analysis with centromeric/telomeric PNA probes could become the standard method for biological dosimetry in radiation emergency medicine. (C) 2012 by Radiation Research Society
