The G-protein alpha subunit CgGa1 mediates growth, sporulation, penetration and pathogenicity in Colletotrichum gloeosporioides
The G-protein alpha subunit CgGa1 mediates growth, sporulation, penetration and pathogenicity in Colletotrichum gloeosporioides
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G 蛋白 α 亚基 CgGa1 介导胶孢炭疽菌的生长、孢子形成、渗透和致病性
DOI：
10.1016/j.micpath.2021.105254
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发表时间：
2021-10-23
影响因子：
3.8
通讯作者：
Liu, Zhiqiang
中科院分区：
文献类型：
--
作者：
Li, Xiaoyu;Ke, Zhijian;Liu, Zhiqiang
胶孢炭疽菌（Colletotrichum gloeosporioides）是引起橡胶炭疽病的主要病原菌，给天然橡胶产业带来巨大的经济损失。异源三聚体G蛋白在丝状真菌的信号转导中起着重要作用，G α亚基是G蛋白的主要组成部分。在这项研究中，我们的特点是第一组G α亚基CgGa 1在C。gloeosporioides是梨孢霉中MagB的同源物。CgGa1编码353个氨基酸的蛋白质，具有G_α结构域。CgGa1的缺失导致营养生长和分生孢子产量降低，并且突变体不能产生子实体。CgGa1缺失突变体的孢子萌发和附着胞形成也显著降低。此外，该突变体的穿透力明显不足，完全丧失了毒力。转录组分析表明，CgGa 1可以影响与碳水化合物代谢、氨基酸代谢、信号转导等相关基因的表达。胶孢菌
Colletotrichum gloeosporioides is the main pathogen causing rubber anthracnose, which brings huge economic loss to the natural rubber industry. Heterotrimeric G proteins play a vital role in signal transduction in filamentous fungi, and G alpha subunits are the major component of G proteins. In this study, we characterize a group I G alpha subunit CgGa1 in C. gloeosporioides as a homolog of MagB in Pyricularia oryzae. CgGa1 encodes a 353-amino acid protein and has a G_alpha domain. Deletion of CgGa1 results in reduced vegetative growth and conidia yield, and the mutant cannot produce a fruiting body. The CgGa1 deletion mutant also exhibits decreased conidial germination and appressorium formation significantly. Moreover, the mutant has an obvious deficiency in penetration and loses its virulence completely. Transcriptome analysis showed that CgGa1 could affect the expression of many genes related to carbohydrate metabolism, amino acid metabolism and signal transduction, etc. In conclusion, CgGa1 regulates growth, asexual and sexual sporulation, appressorium formation, penetration and pathogenicity of C. gloeosporioides.