PREDICTING THE ORIENTATION OF EUKARYOTIC MEMBRANE-SPANNING PROTEINS
PREDICTING THE ORIENTATION OF EUKARYOTIC MEMBRANE-SPANNING PROTEINS
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DOI：
10.1073/pnas.86.15.5786
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发表时间：
1989-08-01
影响因子：
11.1
通讯作者：
LODISH, HF
中科院分区：
文献类型：
--
作者：
HARTMANN, E;RAPOPORT, TA;LODISH, HF
我们已经制定了一条规则，用于预测在粗面内质网上合成的真核跨膜蛋白中第一个内部信号锚序列的方向。第一个内部信号锚两侧15个残基的电荷差异决定了它的方向，其中带正电荷较多的部分朝向细胞质。在多次跨膜的蛋白质中，所有后续跨膜片段的方向将由最靠近N端的那个跨膜片段的方向决定。
We have developed a rule to predict the orientation of the first internal signal-anchor sequence in eukaryotic transmembrane proteins synthesized on the rough endoplasmic reticulum. The difference in the charges of the 15 residues flanking the first internal signal-anchor determines its orientation, with the more positive portion facing the cytosol. In proteins that span the membrane more than once, the orientation of all subsequent transmembrane segments would be determined by that of the most N-terminal one.