Enhancement of mite allergen-induced eosinophil infiltration in the murine airway and local cytokine/chemokine expression by asian sand dust
Enhancement of mite allergen-induced eosinophil infiltration in the murine airway and local cytokine/chemokine expression by asian sand dust
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DOI：
10.1080/15287390500470833
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发表时间：
2006-08-15
影响因子：
2.6
通讯作者：
Shibamoto, Takayuki
中科院分区：
文献类型：
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作者：
Ichinose, Takamichi;Sadakane, Kaori;Shibamoto, Takayuki
目前还没有关于沙尘对室内过敏原（如屋尘螨）引起的过敏性哮喘的影响的数据。本研究旨在阐明亚洲沙尘对螨过敏原粉尘螨（Dermatophagoides farinae）的作用。用毛乌素沙漠（内蒙古）（SD-1）和腾格里沙漠（中国）（SD-2）的沙尘在小鼠肺中诱导嗜酸性粒细胞炎症。ICR小鼠经气管内注射生理盐水;单独SD-1;单独SD-2; D. farinae单独; farinae + SD-1; D.粉尘+ SD-2，4次，间隔2周。两种沙尘均能增强气道嗜酸性粒细胞浸润，沿着与D.花粉SD-2诱导的嗜酸性粒细胞浸润程度大于SD-1。SD-1含有较高量的β-葡聚糖，在有或无D. farinae，但SD-2没有。BALF中白细胞介素（IL）-5、嗜酸性粒细胞趋化因子和单核细胞趋化蛋白水平协同或累积升高与D。farinae与D. farinae + SD-2处理组比D.花粉+ SD-1。提示BALF中细胞因子和趋化因子的增加在D.粉尘+沙尘。SD-1处理小鼠中嗜酸性粒细胞浸润减少可能是由于微生物材料（如β-葡聚糖）诱导的干扰素-γ抑制Th-2细胞因子和嗜酸性粒细胞活化趋化因子所致。
Data on the effects of sand dust toward allergic asthma produced by indoor allergens, such as house dust mites, are not currently available. This study was undertaken to clarify the role of Asian sand dust on mite allergen, Dermatophagoides farinae (D. farinae)-induced eosinophilic inflammation in the murine lung, using sand dusts from the Maowusu Desert (Inner Mongolia) (SD-1) and the Tengger Desert (China) (SD-2). ICR mice were intratracheally administered saline; SD-1 alone; SD-2 alone; D. farinae alone; D. farinae + SD-1; and D. farinae + SD-2, 4 times at 2-wk intervals. The two sand dusts enhanced infiltration of eosinophil in the airway, along with goblet-cell proliferation related to D. farinae . The degree of eosinophil infiltration induced with SD-2 was greater than with SD-1. The SD-1, which contained higher amounts of beta-glucan, increased the expression of interferon (IFN)-gamma in bronchoalveolar lavage fluids (BALF) with or without D. farinae , but SD-2 did not. Synergistically or cumulatively elevated levels of interleukin (IL)-5, eotaxin, and monocyte chemotactic protein in BALF related to D. farinae were higher with D. farinae + SD-2 than with D. farinae + SD-1. These results suggest that increased cytokine and chemokines in BALF play an important role in the enhancement of eosinophil infiltration in the airway induced by D. farinae + sand dusts. The reduced eosinophil infiltration in the SD-1-treated mice could be due to suppression of Th-2 cytokine and eotaxin via interferon-gamma induced by microbial materials, such as beta-glucan.