INFLUENZA-VIRUS RNA IN THE LUNG AND LYMPHOID-TISSUE OF IMMUNOLOGICALLY INTACT AND CD4-DEPLETED MICE
INFLUENZA-VIRUS RNA IN THE LUNG AND LYMPHOID-TISSUE OF IMMUNOLOGICALLY INTACT AND CD4-DEPLETED MICE
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DOI：
10.1099/0022-1317-72-7-1695
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发表时间：
1991-07-01
影响因子：
3.8
通讯作者：
DOHERTY, PC
中科院分区：
文献类型：
--
作者：
EICHELBERGER, MC;WANG, ML;DOHERTY, PC
本文对H3N2型甲型流感病毒鼻内感染C57BL/6J小鼠所致肺炎急性期和恢复期的病毒RNA(VRNA)和信使核糖核酸(MRNA)的分布和清除进行了分析。用聚合酶链式反应扩增病毒基因组材料表明，免疫正常的小鼠在感染后第14天，流感血凝素(HA)基因从肺中被清除，而在体外，CD4+T细胞的清除最多延迟了4天。编码HA基因的病毒RNA于48h在区域纵隔淋巴结中首次出现，并分别持续存在至感染完整小鼠和CD4耗竭小鼠的第6天和第10天。在某些情况下，有证据表明vRNA存在于胸腺中，但不存在于肠系膜淋巴结或脾中。此外，vRNA的分布和清除与早期使用病毒分离程序监测定位模式的研究所预测的一样，表明流感病毒基因组缺乏长期持久性。
The distribution and clearance of viral RNA (vRNA) and mRNA has been analysed for the acute and recovery stages of the pneumonia induced by intranasal infection of C57BL/6J mice with H3N2 influenza A viruses. Amplification of viral genomic material by the polymerase chain reaction showed that the influenza haemagglutinin (HA) gene was eliminated from the lungs of immunologically intact mice by day 14 post-infection, whereas in vitro depletion of the CD4+ T cells delayed clearance by at most 4 days. Viral RNA encoding the HA gene was first demonstrated in the regional mediastinal lymph nodes at 48 h, and continued to be present until day 6 or day 10 after infection of the intact and CD4-depleted mice, respectively. Evidence for the presence of vRNA in the thymus, but not in the mesenteric lymph nodes or the spleen, was found in some situations. Otherwise, the distribution and clearance of vRNA was as would be predicted from earlier studies using virus isolation procedures to monitor localization patterns, and shows a lack of long-term persistence of the influenza virus genome.