Evidence for an increased geographical distribution of Dermacentor reticulatus in Germany and detection of Rickettsia sp RpA4
Evidence for an increased geographical distribution of Dermacentor reticulatus in Germany and detection of Rickettsia sp RpA4
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DOI：
10.1016/j.ijmm.2006.01.013
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发表时间：
2006-05-01
影响因子：
4.1
通讯作者：
Schettler, Elvira
中科院分区：
文献类型：
--
作者：
Dautel, Hans;Dippel, Cornelia;Schettler, Elvira
进行了两项研究以阐明网状革蜱在德国的当前分布。在2003年的第一次调查中，对来自柏林和勃兰登堡州171个地点的365只犬进行了蜱虫筛查，并采用标记法对犬经常访问的相应户外地点进行了寻找宿主蜱虫的搜索。从犬中共取出1155只蜱虫。其中以蓖麻硬蜱为主（88.5%），其次为杜氏硬蜱。网脉甲（9.1%）和I.六边形占2.4%。总共有222只狗携带I.蓖麻（60.8%）、41株D.网脉I. reticulatus（11.2%）和15株I.六角形蜱占4.1%。根据盾叶指数的测定，去除的I。ricinus和D.网织红蜘蛛分别饲喂犬的平均时间为4.0天和4.5天。感染D.网纹革蜱生活在26个不同的地点，所有以前未知的Dermacentor网站。其中7个地点随后可以通过标记植被中的蜱虫来确认。在第二项研究中，在2004年秋季狩猎季节，共有721只鹿在德国160个地区的201个不同地点被射杀。14个调查点的23头鹿（3.2%）被感染。网状的因此，红鹿携带D.网纹蜱发现的地点中只有两个已经被称为D。而在勃兰登堡、萨克森安哈尔特、黑森和巴伐利亚的所有其他地点都是未知的。两项研究的结果均表明D.目前，网纹虫在德国出现的地点比以前所知的要多得多，因此很可能已经扩大了其分布范围。此外，共有135个D。用PCR方法对从鹿体中分离的网状绦虫进行犬巴贝虫和立克次体的筛选。共31个D。网状群立克次体阳性率为23%。测序结果显示，在所有情况下，与首次从俄罗斯扇头蜱属蜱中分离的菌株RpA4具有100%的同一性。(c)2006年Elsevier GmbH。All rights reserved.
Two studies were performed to elucidate the current distribution of the tick Dermacentor reticulatus in Germany. In the first one in 2003, a total of 365 dogs from 171 sites in the states of Berlin and Brandenburg was screened for ticks, and the corresponding outdoor sites that the dogs usually visited were searched for host-seeking ticks by the flagging method. A total of 1155 ticks was removed from the dogs. The majority were Ixodes ricinus (88.5%), followed by D. reticulatus (9.1%) and I. hexagonus (2.4%). Altogether, 222 dogs carried I. ricinus (60.8%), 41 D. reticulatus (11.2%) and 15 I. hexagonus (4.1%) ticks. Based on scutal index determination, the removed I. ricinus and D. reticulatus had been feeding on the dogs for a mean of 4.0 and 4.5 days, respectively. The dogs infested with D. reticulatus lived at 26 different sites, all previously unknown as Dermacentor sites. Seven of the sites could be confirmed subsequently by flagging the vegetation for ticks. In the second study, a total of 721 deer was shot at 201 different sites from a total of 160 districts all over Germany during the autumn hunting season 2004. A total of 23 deer (3.2%) originating from 14 sites was infested with D. reticulatus. Hereby, significantly more red deer (Cervus elaphus elaphus) than roe deer (Capreolus capreolus) or fallow deer (Dama dama) harboured D. reticulatus ticks. Only two of the sites found had already been known as D. reticulatus areas, whereas all other sites in Brandenburg, Saxony Anhalt, Hesse and Bavaria had been unknown. The results of both studies show that D. reticulatus presently occurs at far more sites than previously known in Germany and thus most likely has expanded its range. Additionally, a total of 135 D. reticulatus removed from deer was screened for Babesia canis and Rickettsia sp. by PCR. A total of 31 D. reticulatus (23%) were positive for Rickettsia. Sequencing revealed in all cases 100% identity with the strain RpA4 that was first isolated from Rhipicephalus ticks in Russia. (c) 2006 Elsevier GmbH. All rights reserved.