A comprehensive definition of the major antibody specificities in polyclonal rabbit antithymocyte globulin.
A comprehensive definition of the major antibody specificities in polyclonal rabbit antithymocyte globulin.
复制标题
多克隆兔抗胸腺细胞球蛋白主要抗体特异性的全面定义。
DOI：
10.1097/00007890-199403150-00010
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发表时间：
1994
期刊：
影响因子：
6.2
通讯作者：
Thomas,JM
中科院分区：
文献类型：
--
作者：
Rebellato,LM;Gross,U;Verbanac,KM;Thomas,JM
兔抗胸腺细胞球蛋白（RATG）对同种异体移植受者的有效免疫抑制作用已被认识多年。RATG的一些抗体特异性和免疫调节作用已被描述，但对RATG成分的全面定义尚未有报道。在这项研究中，我们确定了23个在不同临床RATG批次中一致的特异性，代表了RATG中的主要抗体特异性。这些特异性是通过免疫沉淀/凝胶电泳和抗体阻断/流式细胞术方法确定的。对RATG抗体进行半定量分析的滴度研究显示，滴度最高的抗体主要针对CD6、CD16、CD18、CD28、CD38、CD40和CD58（滴度>:4000），其中大部分不是T细胞特异性抗体。相比之下，在恒河猴移植受体血浆中持续时间最长的RATG抗体是针对CD3、CD4、CD8、CDlla、CD40、CD45、CD54和i类的抗体。这些抗体针对信号转导和粘附分子，在淋巴细胞恢复的早期就存在。我们认为，这些抗体在体内的持续存在与RATG治疗后循环T细胞的长时间能量和移植中不寻常的效力和复杂的免疫效应直接相关。
The potent immunosuppressive action of rabbit antithymocyte globulin (RATG) in allotransplant recipients has been recognized for many years. Some of the antibody specificities and immunoregulatory effects of RATG have been described, but a comprehensive definition of RATG components has not been reported previously. In this study, we have identified 23 specificities that are consistent among different clinical RATG batches and represent the major antibody specificities in RATG. These specificities were defined by immunoprecipitation/gel electrophoresis and also antibody blocking/flow cytometry methods. Titration studies performed for semiquantitative analysis of RATG antibodies showed that the antibodies present in highest titer were directed to CD6, CD16, CD18, CD28, CD38, CD40, and CD58 (titer> 1: 4000), most of which are not T cell-specific antibodies. In contrast, the RATG antibodies that persisted the longest in vivo in the plasma of rhesus monkeys transplant recipients are antibodies to CD3, CD4, CD8, CDlla, CD40, CD45, CD54, and class I. These antibodies, which are directed at signal transduction and adhesion molecules, were present during the early period of lymphocyte recovery. We suggest that the persistence of these antibodies in vivo is directly related to the prolonged anergy of circulating T cells after RATG treatment and to the unusual potency and complex tapestry of immunological effects in transplantation.