Antibody-mediated depletion of tumor necrosis factor-alpha impairs pulmonary host defenses to Legionella pneumophila.
Antibody-mediated depletion of tumor necrosis factor-alpha impairs pulmonary host defenses to Legionella pneumophila.
复制标题
抗体介导的肿瘤坏死因子-α 耗竭会损害肺部宿主对嗜肺军团菌的防御。
DOI：
10.1086/516530
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Nelson,S
中科院分区：
文献类型：
--
作者：
Skerrett,SJ;Bagby,GJ;Schmidt,RA;Nelson,S
Tumor necrosis factor-α (TNF-α) has been shown to stimulate the resistance of alveolar macrophages and neutrophils toLegionella pneumophilain vitro. To determine whether endogenous TNF-α is necessary for host defense against legionellosis in vivo, anti-TNF-α IgG or control IgG was administered to rats exposed to aerosolizedL. pneumophila. Treatment with anti-TNF-aneutralized >90% of the intrapulmonary TNF-α response to infection, resulting in persistent pneumonitis and failure to clearL. pneumophilafrom the lungs. Depletion of TNF-α limited the recruitment of mononuclear cells to the lungs and resulted in a progressive increase in the proportion of alveolar macrophages that were infected; neutrophil recruitment and phagocytosis were not impaired. Both systemic and intrapulmonary IFN-γ levels were significantly higher in rats depleted of TNF-α. These observations indicate that TNF-α is required for the prompt resolution of pneumonic legionellosis and point to a direct role for TNF-α in the activation of phagocytes.
