A New EPR Oximetry Protocol to Estimate the Tissue Oxygen Consumption In Vivo
A New EPR Oximetry Protocol to Estimate the Tissue Oxygen Consumption In Vivo
复制标题
DOI：
10.1667/rr1448.1
复制
发表时间：
2009-08-01
影响因子：
3.4
通讯作者：
Gallez, Bernard
中科院分区：
文献类型：
--
作者：
Diepart, Caroline;Jordan, Benedicte F.;Gallez, Bernard
肿瘤的氧耗率影响肿瘤的氧合和对治疗的反应。建立了一种无创测量组织耗氧量的EPR新方法。该方案基于在Carbogen挑战期间对Po(2)的测量。采用如下顺序：(1)空气呼吸时的基础值；(2)二氧化碳呼吸时组织含氧量的饱和度；(3)切换回空气呼吸。假设氧饱和后恢复到基础值的动力学将主要由组织耗氧量决定。这一挑战应用于甲状腺功能亢进症小鼠(由L-甲状腺素慢性治疗产生)和对照组小鼠，因为已知甲状腺功能亢进症显著影响肿瘤和肌肉细胞的耗氧率。甲亢小鼠的肌肉和肿瘤细胞比对照小鼠的肌肉和肿瘤细胞消耗氧气的速度更快，这与体外研究的结果一致。L-甲状腺素治疗不影响肿瘤的血流灌注。这种方法给出的指标可以合理地归因于局部耗氧量，并具有适用于活体研究的独特优势。(C)2009年，由辐射研究学会提供
The oxygen consumption rate in tumors affects tumor oxygenation and response to therapies. A new EPR method was developed to measure tissue oxygen consumption non-invasively. The protocol was based on the measurement of pO(2) during a carbogen challenge. The following sequence was used: (1) basal value during air breathing; (2) saturation of tissue with oxygen by carbogen breathing; (3) switch back to air breathing. The assumption was that the kinetics of the return to the basal value after oxygen saturation would be governed mainly by tissue oxygen consumption. This challenge was applied in hyperthyroid mice (generated by chronic treatment with L-thyroxine) and control mice because hyperthyroidism is known to dramatically affect the oxygen consumption rate of tumor and muscle cells. Muscle and tumor cells from the hyperthyroid mice consumed oxygen faster than muscle and tumor cells from the control mice, which is consistent with the results of in vitro studies. Tumor perfusion was not affected by the treatment with L-thyroxine. This method gives an index that may reasonably be ascribed to the local oxygen consumption and has the unique advantage of being adaptable to in vivo studies. (C) 2009 by Radiation Research Society