Characterisation of Phosphate Solubilising Bacteria in Sandy Loam Soil Under Chickpea Cropping System
Characterisation of Phosphate Solubilising Bacteria in Sandy Loam Soil Under Chickpea Cropping System
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DOI：
10.1007/s12088-011-0209-z
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发表时间：
2011-08
影响因子：
3
通讯作者：
Machiavelli Singh;bullet N Tejo Prakash
中科院分区：
文献类型：
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作者：
Machiavelli Singh;bullet N Tejo Prakash
为探讨溶磷细菌（PSB）在农业土壤磷循环中的作用，从Patiala（旁遮普州）鹰嘴豆种植区的桑迪壤土中分离到一株溶磷细菌。在Pikovskaya琼脂平板上分离了总共31个显示增溶活性的细菌分离株。选择有效的磷酸盐增溶剂用于进一步表征。经16 SrDNA部分序列分析，这些菌株分别属于假单胞菌属和沙雷氏菌属。ERIC-PCR为基础的指纹进行了跟踪的PSB在鹰嘴豆地块下的这些菌株的大规模接种过程中引入的人口的生存。结果表明，土壤磷酸酶活性与溶磷菌数量呈正相关（r2= 0.853），与速效磷呈正相关（r2= 0.730）。鉴定和表征土壤光合细菌对植物生长的有效促进作用拓宽了可用于田间应用的磷增溶剂的范围。
With the aim to explore the possible role of phosphate-solubilizing bacteria (PSB) in phosphorus (P) cycling in agricultural soils, we isolated PSB inhabiting naturally in the sandy loam soils under chickpea cropping of Patiala (Punjab State). A total of 31 bacterial isolates showing solubilizing activities were isolated on Pikovskaya agar plates. The potent phosphate solubilizers were selected for further characterization. These isolates were shown to belong to the generaPseudomonasandSerratiaby partial sequencing analysis of their respective 16S rDNA genes. ERIC-PCR based fingerprinting was done for tracking the survival of introduced populations of the PSB during mass inoculation of these strains under chickpea plots. The results showed positive correlation (r2= 0.853) among soil phosphatase activity and phosphate solubilizers population, which was also positively correlated (r2= 0.730) to available phosphorus. Identification and characterization of soil PSB for the effective plant growth-promotion broadens the spectrum of phosphate solubilizers available for field application.