Characterisation of a South African human astrovirus as type 8 by antigenic and genetic analyses
Characterisation of a South African human astrovirus as type 8 by antigenic and genetic analyses
复制标题
DOI：
10.1002/jmv.1044
复制
发表时间：
2001-07-01
影响因子：
12.7
通讯作者：
Matson, DO
中科院分区：
文献类型：
--
作者：
Taylor, MB;Walter, J;Matson, DO
人星状病毒（HAstV），可以使用型特异性兔抗血清的免疫测定的基础上，分为八个血清型与基因型相关。已经描述了非常少的HAstV 8型分离株，并且关于HAstV 8型（HAstV-8）和HAstV 1型（HAstV-1）至7型（HAstV-7）之间的抗原和遗传关系的可用数据很少。通过免疫电子显微镜和酶免疫测定法对来自南非腹泻儿科患者的野生型HAstV进行抗原性分析，并在ORF 1a，ORF 1b和ORF 2的选定区域进行遗传分析，并将其表征为HAstV-8。HAstV-8株与HAstV-4在衣壳基因的5 '端和ORF 1a和Ib具有最大的同源性，与HAstV-5在衣壳区的3 '端具有最大的同源性。这项研究证实，抗原和遗传分析，HAstV-8代表了一个独特的抗原和基因型，是第一次报告的HAstV-8从住院的儿科腹泻患者在南部非洲。J.疯了。Virol. 64：256-261，2001. (C)2001 Wiley-liss，Inc.
Human astroviruses (HAstV) can, on the basis of immunoassays using type-specific rabbit antisera, be classified into eight serotypes that correlate with genotypes. Very few isolates of HAstV type 8 have been described and there is a paucity of data available with regard to the antigenic and genetic relationships between HAstV type 8 (HAstV-8) and HAstV types 1 (HAstV-1) to 7 (HAstV-7). A wild-type HAstV from a South African paediatric patient with diarrhoea was analysed antigenically, by immune electron microscopy and enzyme immunoassay, and genetically in selected regions of the ORF1a, ORF1b and ORF2 and characterised as a HAstV-8. This HAstV-8 strain exhibited greatest homology with HAstV-4 in the 5 ' end of the capsid gene and ORF1a and Ib, and greatest homology with HAstV-5 in the 3 ' end of the capsid region. This study confirms, by both antigenic and genetic analyses, that HAstV-8 represents a distinct antigenic and genotype and is the first report of a HAstV-8 from a hospitalised paediatric patient with diarrhoea in southern Africa. J. Mad. Virol. 64:256-261, 2001. (C) 2001 Wiley-liss, Inc.