Fecal microbial communities of healthy adult dogs fed raw meat-based diets with or without inulin or yeast cell wall extracts as assessed by 454 pyrosequencing
Fecal microbial communities of healthy adult dogs fed raw meat-based diets with or without inulin or yeast cell wall extracts as assessed by 454 pyrosequencing
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DOI：
10.1111/1574-6941.12081
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发表时间：
2013-06-01
影响因子：
4.2
通讯作者：
Swanson, Kelly S.
中科院分区：
文献类型：
--
作者：
Beloshapka, Alison N.;Dowd, Scot E.;Swanson, Kelly S.
我们的目的是用454焦磷酸测序法确定添加或不添加菊粉或酵母细胞壁提取物(YCW)的生肉饲料对犬粪便微生物群落的影响。6只健康成年雌性比格犬(5.5+/-0.5岁；8.5+/-0.5 kg)按拉丁方设计随机分为6组：(1)牛肉对照组；(2)牛肉+1.4%菊粉；(3)牛肉+1.4%YCW；(4)鸡对照组；(5)鸡+1.4%菊粉；(6)鸡+1.4%YCW。在适应14天后，在每个时期的第15天或第16天收集新鲜粪便样本。提取粪便基因组DNA，扩增出16S rRNA基因扩增产物，进行454焦磷酸测序和qPCR检测。主要的粪便细菌门包括梭杆菌、纤毛杆菌、拟杆菌和变形杆菌。以牛肉为基础的日粮增加(P
Our objective was to determine the effects of feeding raw meatbased diets with or without inulin or yeast cell wall extract (YCW) on fecal microbial communities of dogs using 454 pyrosequencing. Six healthy female adult beagles (5.5 +/- 0.5years; 8.5 +/- 0.5kg) were randomly assigned to six test diets using a Latin square design: (1) beef control; (2) beef+1.4% inulin; (3) beef+1.4% YCW; (4) chicken control; (5) chicken+1.4% inulin; and (6) chicken+1.4% YCW. Following 14days of adaptation, fresh fecal samples were collected on day 15 or day 16 of each period. Fecal genomic DNA was extracted and used to create 16S rRNA gene amplicons, which were subjected to 454 pyrosequencing and qPCR. Predominant fecal bacterial phyla included Fusobacteria, Firmicutes, Bacteroidetes, and Proteobacteria. Beef-based diets increased (P