Intrapulmonary Delivery of CpG-ODN Microdroplets Provides Protection Against Escherichia coli Septicemia in Neonatal Broiler Chickens
Intrapulmonary Delivery of CpG-ODN Microdroplets Provides Protection Against Escherichia coli Septicemia in Neonatal Broiler Chickens
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DOI：
10.1637/11684-060617-reg.1
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发表时间：
2017-12-01
期刊：
影响因子：
1.4
通讯作者：
Gomis, Susantha
中科院分区：
文献类型：
--
作者：
Goonewardene, Kalhari Bandara;Popowich, Shelly;Gomis, Susantha
含未甲基化胞嘧啶磷酸二酯鸟嘌呤（CpG）基序的人工合成寡脱氧核苷酸（CpG-ODN）是一种有效的免疫刺激剂，能有效地抑制包括家禽在内的多种动物的病毒、细菌和原生动物疾病。我们最近已经证明，在卵内注射CpG-ODN赋予保护新生鸡对细菌败血症。本研究旨在探讨CpG-ODN微滴无针肺内给药对新生雏鸡大肠杆菌感染的有效性。在本研究中，我们总共使用了880只鸡，每组保留40只鸡。在孵化的第1天，通过IPL向雏鸡递送CpG-ODN或盐水。3d后，用13104 CFU和13105 CFU两个剂量的E.杆菌与盐水处理组相比，通过IPL途径用CpGODN处理的雏鸡具有显著较低的临床体征和细菌负荷（P < 0.05）。CpG-ODN处理组对E.大肠杆菌败血症。我们观察了IPL CpG-ODN对小鸡的免疫保护作用，其具有剂量和暴露时间依赖性。我们发现，IPL递送CpG-ODN可以早在6小时诱导保护性免疫，其至少在治疗后第5天保持有效。此外，IPL递送CpG-ODN对长达42日龄的生长或死亡率没有不利影响。基于这些发现，可以表明，CpG-ODN通过IPL途径递送可以是抗生素的有希望的替代方案，用于在新生儿生命的关键第一周期间诱导雏鸡的保护性免疫。
Synthetic oligodeoxynucleotides (ODN) containing unmethylated cytosine phosphodiester guanine (CpG) motifs (CpG-ODN) are effective immunostimulatory agents against a variety of viral, bacterial, and protozoan diseases in different animals including poultry. We have recently demonstrated that in ovo injection of CpG-ODN confers protection in neonatal chickens against bacterial septicemias. The objective of this study was to investigate the effectiveness of needle-free intrapulmonary (IPL) delivery of CpG-ODN microdroplets against Escherichia coli infection in neonatal chicks. In the present study, we used 880 chicks in total keeping 40 chicks per group. Chicks were delivered CpG-ODN or saline by IPL at the day 1 of hatch. Three days later, chicks were challenged with two doses (1310 4 CFU, n = 20 or 1310 5 CFU, n = 20) of E. coli. Chicks treated with CpGODN by the IPL route had significantly lower clinical signs and bacterial load compared to the group treated with saline (P < 0.05). CpG-ODN-treated groups were significantly protected against E. coli septicemia. We observed dose-and exposure timedependent immunoprotective effects of IPL CpG-ODN in chicks. We found that IPL delivery of CpG-ODN can induce protective immunity as early as 6 hr that remains effective at least until day 5 post-treatment. Moreover, there were no adverse effects of IPL delivery of CpG-ODN on growth or mortality up to 42 days of age. Based on these findings, it can be suggested that CpG-ODN delivery by IPL route can be a promising alternative to antibiotics for inducing protective immunity in chicks during the critical first week of neonatal life.