The Drosophila pericentrin-like protein is essential for cilia/flagella function, but appears to be dispensable for mitosis.
The Drosophila pericentrin-like protein is essential for cilia/flagella function, but appears to be dispensable for mitosis.
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DOI：
10.1083/jcb.200402130
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发表时间：
2004-06-07
影响因子：
7.8
通讯作者：
Raff, Jordan W
中科院分区：
文献类型：
--
作者：
Martinez-Campos, Maruxa;Basto, Renata;Baker, James;Kernan, Maurice;Raff, Jordan W
中心体由一对中心粒组成，中心粒周围包裹着无定形的中心粒周围物质（PCM）。含有Pericentrin/AKAP 450中心体靶向（PACT）结构域的蛋白质已经涉及将几种蛋白质募集到PCM。我们发现，唯一的PACT结构域蛋白在果蝇（果蝇pericentrin样蛋白[D-PLP]）与中心粒和PCM，是必不可少的有效的中心体招聘的所有六个PCM组件，我们测试。然而，令人惊讶的是，在d-plp突变细胞的有丝分裂过程中，所有六种PCM组分最终都被募集到中心体，并且有丝分裂没有受到显著干扰。尽管能存活，d-plp突变果蝇是严重不协调的，这种表型通常与机械感觉神经元功能缺陷有关。我们发现，这些神经元的感觉纤毛畸形和d-plp突变体的神经元是无功能的。此外，突变精子中的鞭毛是不运动的。因此，D-PLP对于果蝇中功能性纤毛和鞭毛的形成至关重要。
Centrosomes consist of a pair of centrioles surrounded by an amorphous pericentriolar material (PCM). Proteins that contain a Pericentrin/AKAP450 centrosomal targeting (PACT) domain have been implicated in recruiting several proteins to the PCM. We show that the only PACT domain protein in Drosophila (the Drosophila pericentrin-like protein [D-PLP]) is associated with both the centrioles and the PCM, and is essential for the efficient centrosomal recruitment of all six PCM components that we tested. Surprisingly, however, all six PCM components are eventually recruited to centrosomes during mitosis in d-plp mutant cells, and mitosis is not dramatically perturbed. Although viable, d-plp mutant flies are severely uncoordinated, a phenotype usually associated with defects in mechanosensory neuron function. We show that the sensory cilia of these neurons are malformed and the neurons are nonfunctional in d-plp mutants. Moreover, the flagella in mutant sperm are nonmotile. Thus, D-PLP is essential for the formation of functional cilia and flagella in flies.