Day-night and depth differences in haemolymph melatonin of the Norway lobster, Nephrops norvegicus (L.)
Day-night and depth differences in haemolymph melatonin of the Norway lobster, Nephrops norvegicus (L.)
复制标题
DOI：
10.1016/j.dsr.2009.06.001
复制
发表时间：
2009-10-01
影响因子：
2.4
通讯作者：
Sarda, F.
中科院分区：
文献类型：
--
作者：
Aguzzi, J.;Sanchez-Pardo, J.;Sarda, F.
很少有研究来量化和了解褪黑激素在无脊椎动物中的作用。尤其是甲壳类动物和深海动物。在这项研究中，我们研究了穴居十足类甲壳动物挪威海藻（Nephropsnorvegicus，L.）在暴露于单色蓝光（480 nm）周期和 10 和 0.1 lx 黑暗周期期间血淋巴褪黑激素的昼夜差异。这些不同的强度条件模拟了陆架深度（80-100 m）和斜坡深度（300-400 m）的照明，这些龙虾种群主要发现于西地中海。我们的目标是： (a) 使用液相色谱/串联质谱 (LC-MS/MS) 和荧光高效液相色谱 (HPLC) 验证该物种的血淋巴中是否存在褪黑激素； (b) 研究褪黑激素浓度的昼夜变化与运动节律之间的关系，以检验前者是否影响行为。褪黑激素在 LC-MS/MS 中通过 Q1 和 Q3 质量峰在 3.7 分钟的洗脱时间进行鉴定，并且还通过 HPLC 进行检测。研究发现，ION 下的褪黑激素浓度（4.8 +/- 5.3 ng ml(-1)）比 0.1 lx 下的褪黑激素浓度（0.06 +/- 0.03 ng ml(-1)）高两个数量级。此外，白天 10 lx 的增加在 0.1 lx 中不存在。当比较暴露于两种光周期模式的动物的运动节律时，发现昼夜周期性得以保留，但活动时间从夜间转移到白天。将这些数据外推到现场，我们将结果解释为运动活动在水深范围内保留其昼夜特征，但不保留其相位和幅度，其中血淋巴褪黑素降低其浓度和节律波动。 (C) 2009 Elsevier Ltd. 保留所有权利。
Few studies have been conducted to quantify and understand the role of melatonin in invertebrates. and particularly in crustaceans and in deep-sea animals. In this study, we examined day-night differences in haemolymph melatonin of the burrowing decapod crustacean Nephrops norvegicus (L.) during exposure to cycles of monochromatic blue light (480 nm) and darkness cycles of 10 and 0.1 lx. These differential intensity conditions simulate illumination at the depth of the shelf (80-100 m) and of the slope (300-400 m), where these lobster populations are chiefly found in the Western Mediterranean Sea. Our objectives were: (a) to verify the presence of melatonin in the haemolymph of this species using liquid chromatography/tandem mass spectrometry (LC-MS/MS) and fluorescence HPLC (HPLC); and (b) to study the relationship between diel variations in melatonin concentration and locomotor rhythms, in order to examine whether the former influences behaviour. Melatonin was identified in LC-MS/MS by Q1 and Q3 mass peaks at an elution time of 3.7 min, and it was also detected by HPLC. Melatonin concentration was found to be two orders of magnitude higher at ION (4.8 +/- 5.3 ng ml(-1)) than at 0.1 lx (0.06 +/- 0.03 ng ml(-1)). Also, the increase at daytime in 10 lx was absent in 0.1 lx. When the locomotor rhythm of animals exposed to both photoperiod regimes was compared, the diel periodicity was found to be preserved, but the timing of activity shifted from night to day. Extrapolating these data to the field, we interpret our results to mean that locomotor activity preserves its diel character, but not its phase and amplitude, in a bathymetric range where haemolymph melatonin reduces its concentration and rhythmic fluctuation. (C) 2009 Elsevier Ltd. All rights reserved.