The L2 open reading frame of human papillomavirus type 1a encodes a minor structural protein carrying type-specific antigens
The L2 open reading frame of human papillomavirus type 1a encodes a minor structural protein carrying type-specific antigens
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1a 型人乳头瘤病毒的 L2 开放阅读框编码携带类型特异性抗原的次要结构蛋白
作者：
C. A. Komly;F. Breitburd;O. Croissant;R. Streeck
由乳头瘤病毒基因组的开放阅读框编码的蛋白质和在纯化的病毒粒子中检测到的次要多肽仍然不清楚。在此，我们通过其在大肠杆菌中的表达表明，人乳头瘤病毒1a型的开放阅读框架L2编码了一种Mr 76,000的次要结构蛋白。针对截短的L2-β-半乳糖苷酶融合蛋白(其中L2的保守N-末端缺失)所产生的抗血清是针对人乳头瘤病毒1型病毒粒子的类型，并且在高稀释度下具有活性。因此，L2编码型特异性抗原的表达为乳头瘤病毒的鉴定提供了一种强有力的新工具。
The proteins encoded by the open reading frames of papillomavirus genomes and the minor polypeptides detected in purified virions are still poorly defined. We show here by its expression in Escherichia coli that the open reading frame L2 of human papillomavirus type 1a codes for a minor structural protein of Mr 76,000. Antisera raised against a truncated L2-beta-galactosidase fusion protein in which the conserved N-terminal region of L2 is missing are type specific for human papillomavirus type 1 virions and are reactive at high dilutions. Expression of the L2-encoded type-specific antigens thus provides a powerful new tool for the identification of papillomaviruses.
β-半乳糖苷酶基因融合体用于分析大肠杆菌和酵母中的基因表达。
影响因子：
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作者：
Casadaban,MJ;Martinez-Arias,A;Shapira,SK;Chou,J
通讯作者：
Chou,J