Genomic detection of new yeast pre-mRNA 3′-end-processing signals
Genomic detection of new yeast pre-mRNA 3′-end-processing signals
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DOI：
10.1093/nar/27.3.888
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发表时间：
1999-02-01
影响因子：
14.9
通讯作者：
Smith, TF
中科院分区：
文献类型：
--
作者：
Graber, JH;Cantor, CR;Smith, TF
为了研究酿酒酵母3‘末端加工信号，通过将表达的序列标记序列与酵母全基因组比对，鉴定出1352个独特的pre-mRNA 3’末端加工位点，对应861个不同的基因。分析了裂解位点附近的核苷酸词频，揭示了信号元件的特征。除了先前识别的加工信号外，还发现了3'端加工信号序列的两个先前未被表征的成分，特别是位于切割位点两侧的富u序列。其中一个，下游富u信号，提供了酵母和高级真核生物的3'端加工机制之间的进一步联系。通过判别函数对完整的3′端加工位点进行分析，支持“上下文”模型，在该模型中，所有四个元素中信号的总有效性决定了加工是否发生。
To investigate Saccharomyces cerevisiae 3'-end-processing signals, a set of 1352 unique pre-mRNA 3'-end-processing sites, corresponding to 861 different genes, was identified by alignment of expressed sequence tag sequences with the complete yeast genome. Nucleotide word frequencies in the vicinity of the cleavage sites were analyzed to reveal the signal element features. In addition to previously recognized processing signals, two previously uncharacterized components of the 3'-end-processing signal sequence were discovered, specifically a predominance of U-rich sequences located on either side of the cleavage site. One of these, the downstream U-rich signal, provides a further link between the 3'-end-processing mechanisms of yeast and higher eukaryotes. Analysis of the complete set of 3'-end-processing sites by means of a discrimination function supports a 'contextual' model in which the sum total effectiveness of the signals in all four elements determines whether or not processing occurs.