Network stability through homeostatic scaling of excitatory and inhibitory synapses following inactivity in CA3 of rat organotypic hippocampal slice cultures
Network stability through homeostatic scaling of excitatory and inhibitory synapses following inactivity in CA3 of rat organotypic hippocampal slice cultures
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DOI：
10.1016/j.mcn.2006.01.009
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发表时间：
2006-04-01
影响因子：
3.5
通讯作者：
Empson, RM
中科院分区：
文献类型：
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作者：
Buckby, LE;Jensen, TP;Empson, RM
稳态可塑性是一种现象，突触强度根据网络接收到的活动而变化。在这里，我们分析了一个稳态可塑性模型中的兴奋性和抑制性突触，在该模型中，NMDA和AMPA/KA谷氨酸受体拮抗剂AP5和CNQX剥夺了大鼠海马片培养的兴奋性突触输入。我们发现，慢性兴奋性突触剥夺产生了一个可兴奋的CA3网络，其中自发兴奋性突触后电位的幅度和频率增加与谷氨酸受体亚单位表达增加以及突触素1和VGLUT1阳性点数和大小增加有关。完整的自发抑制性突触后电位与GABA-A受体α亚单位和GAD65的持续表达以及小白蛋白阳性斑点的增强相一致。在这种稳态可塑性模型中，突触兴奋的放大和快速突触抑制的维持促进了一个可兴奋但稳定的CA3网络。(C)2006 Elsevier Inc.保留所有权利。
Homeostatic plasticity is a phenomenon whereby synaptic strength is scaled in the context of the activity that the network receives. Here, we have analysed excitatory and inhibitory synapses in a model of homeostatic plasticity where rat organotypic hippocampal slice cultures were deprived of excitatory synaptic input by the NMDA and AMPA/KA glutamate receptor antagonists, AP5 and CNQX. We show that chronic excitatory synapse deprivation generates an excitable CA3 network where enhanced amplitude and frequency of spontaneous excitatory post-synaptic potentials were associated with increased glutamate receptor subunit expression and increased number and size of synapsin 1 and VGLUT1 positive puncta. Intact spontaneous inhibitory post-synaptic potentials coincided Nvith persistent expression of the GABA-A receptor alpha subunit and GAD65 and an enhancement of parvalbumin-positive puncta. In this model of homeostatic plasticity, scaling up of synaptic excitation and maintenance of fast synaptic inhibition promote an excitable, but stable, CA3 network. (c) 2006 Elsevier Inc. All rights reserved.