The endocytic receptor megalin binds the iron transporting neutrophil-gelatinase-associated lipocalin with high affinity and mediates its cellular uptake
The endocytic receptor megalin binds the iron transporting neutrophil-gelatinase-associated lipocalin with high affinity and mediates its cellular uptake
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DOI：
10.1016/j.febslet.2004.12.031
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发表时间：
2005-01-31
期刊：
影响因子：
3.5
通讯作者：
Borregaard, N
中科院分区：
文献类型：
--
作者：
Hvidberg, V;Jacobsen, C;Borregaard, N
中性粒细胞-明胶酶相关脂钙蛋白（NGAL）是人类中性粒细胞特异性颗粒的重要蛋白，也由上皮细胞在炎症期间合成。NGAL结合细菌的铁载体，阻止细菌从这个来源获取铁。此外，NGAL可能在原始肾小管上皮细胞形成过程中将铁传递到细胞中发挥重要作用。没有描述NGAL的细胞受体。通过表面等离子体共振分析，我们发现在极化上皮中表达的低密度脂蛋白受体家族成员meggalin与NGAL具有高亲和力。此外，已知表达高水平meggalin的大鼠卵黄囊细胞系通过一种被meggalin抗体完全阻断的机制内吞NGAL。(C) 2004年欧洲生化学会联合会。Elsevier B.V.版权所有。
Neutrophil-gelatinase-associated lipocalin (NGAL) is a prominent protein of specific granules of human neutrophils also synthesized by epithelial cells during inflammation. NGAL binds bacterial siderophores preventing bacteria from retrieving iron from this source. Also, NGAL may be important in delivering iron to cells during formation of the tubular epithelial cells of the primordial kidney. No cellular receptor for NGAL has been described.We show here that megalin, a member of the low-density lipoprotein receptor family expressed in polarized epithelia, binds NGAL with high affinity, as shown by surface plasmon resonance analysis. Furthermore, a rat yolk sac cell line known to express high levels of megalin, endocytosed NGAL by a mechanism completely blocked by an antibody against megalin. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.