MOLECULAR-CLONING AND EXPRESSION OF A 90-KDA DIACYLGLYCEROL KINASE THAT PREDOMINANTLY LOCALIZES IN NEURONS
MOLECULAR-CLONING AND EXPRESSION OF A 90-KDA DIACYLGLYCEROL KINASE THAT PREDOMINANTLY LOCALIZES IN NEURONS
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DOI：
10.1073/pnas.90.16.7598
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发表时间：
1993-08-15
影响因子：
11.1
通讯作者：
KONDO, H
中科院分区：
文献类型：
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作者：
GOTO, K;KONDO, H
从大鼠脑cDNA文库中分离到一个甘油二酯激酶cDNA。该cDNA编码801个氨基酸的蛋白质90，287 Da. This 90-kDa的甘油二酯激酶与先前分离的大鼠80-kDa的甘油二酯激酶在推导的氨基酸序列中显示58%的同一性。EF-手状基序、富含半胱氨酸的锌指样序列和推定的ATP结合位点在这两种激酶之间都是保守的。然而，编码90 kDa激酶的mRNA仅限于有限的神经元群体，如尾壳核，延髓核和嗅结节。此外，90 kDa激酶被发现表现出高磷酸化活性的长链二酰基甘油，并主要与膜馏分时，cDNA转染到COS-7细胞。
A diacylglycerol kinase cDNA was isolated from a rat brain cDNA library. This cDNA encoded an 801-amino acid protein of 90,287 Da. This 90-kDa diacylglycerol kinase showed 58% identity in deduced amino acid sequence with a previously isolated rat 80-kDa diacylglycerol kinase. EF-hand motifs, cysteine-rich zinc-finger-like sequences, and putative ATP-binding sites were all conserved between the two kinase species. However, mRNA encoding the 90-kDa kinase was confined to restricted neuronal populations such as the caudate-putamen, the accumbens nucleus, and the olfactory tubercle. Further, the 90-kDa kinase was found to exhibit high phosphorylation activity for long-chain diacylglycerols and was mainly associated with the membrane fraction when the cDNA was transfected into COS-7 cells.