Preparation of biocompatible wound dressings with long-term antimicrobial activity through covalent bonding of antibiotic agents to natural polymers
Preparation of biocompatible wound dressings with long-term antimicrobial activity through covalent bonding of antibiotic agents to natural polymers
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DOI：
10.1016/j.ijbiomac.2018.09.122
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发表时间：
2019-02-15
影响因子：
8.2
通讯作者：
Shi, Xianai
中科院分区：
文献类型：
--
作者：
Hu, Shengxue;Cai, Xianqun;Shi, Xianai
具有长期抗菌活性的创面敷料是治疗慢性创面感染的理想材料。本论文以环丙沙星(CIP)和硫酸庆大霉素(GS)为抗菌剂，与天然高分子基质复合材料、羧甲基壳聚糖(CMC)和胶原(Col)共价键合，研制了一种新型的抗菌创面敷料。通过抗菌剂与聚合物链形成酰胺键，制备了CMC-Col-CIP和CMC-Col-GS两种抗菌创面敷料。本发明的创面敷料具有高吸水性、良好的水蒸气透过率(WVTR)、适当的机械性能和令人印象深刻的稳定性。通过体外培养人皮肤成纤维细胞(HSF)，证实了敷料的细胞相容性。体内外研究结果表明，两种抗菌创面敷料均具有有效的抗菌作用，并延长了抗菌时间。此外，抗菌敷料还能促进创面愈合、再上皮化、胶原沉积和血管生成。与市面上可买到的银基敷料(Aguacel Ag)相比，它还显示出更优越的伤口愈合效果。该抗菌创面敷料在严重创面愈合、糖尿病足部溃疡等慢性创面修复中具有广阔的应用前景。(C)2018爱思唯尔B.V.保留所有权利。
Wound dressings with long-term antimicrobial activity are highly desired for treatment of chronic wound infections. Herein, the sustained antimicrobial wound dressings were developed by using antibiotic agents, ciprofloxacin HCL (CIP) and gentamicin sulfate (GS), covalent bonding to natural polymer matrix composites, carboxymethyl chitosan (CMC) and collagen (COL). By amide bond formation between antibiotic agents and polymer chains, two antimicrobial wound dressings CMC-COL-CIP and CMC-COL-GS were prepared. The presented wound dressings exhibited high water absorption capacity, excellent water vapor transmission rate (WVTR), appropriate mechanical properties, and impressive stability. Cytocompatibility of the dressings was demonstrated by in vitro human skin fibroblast (HSF) cells culture study. The results of in vitro and in vivo studies indicated that the two antimicrobial wound dressings have effective antimicrobial activity and prolonged antimicrobial period. Furthermore, the antimicrobial dressings could promote the wound healing, reepithelialization, collagen deposition, and angiogenesis. It also displays superiority wound healing effects compared to commercially available silver-based dressings (Aguacel Ag). This work indicates that the prepared antimicrobial wound dressings have great potential application in chronic wound healing, such as severe wound cure and diabetic foot ulcers. (C) 2018 Elsevier B.V. All rights reserved.