Genome-wide identification and comparative analysis of the cation proton antiporters family in pear and four other Rosaceae species
Genome-wide identification and comparative analysis of the cation proton antiporters family in pear and four other Rosaceae species
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DOI：
10.1007/s00438-016-1215-y
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发表时间：
2016-05
影响因子：
3.1
通讯作者：
Hongshen Zhou;K. Qi;Xing Liu;Hao Yin;Peng Wang;Jianqing Chen;Juyou Wu;Shaoling Zhang
中科院分区：
文献类型：
--
作者：
Hongshen Zhou;K. Qi;Xing Liu;Hao Yin;Peng Wang;Jianqing Chen;Juyou Wu;Shaoling Zhang
一价阳离子质子反向转运蛋白（CPAs）通过调节细胞内离子和pH的平衡，在植物营养、生长发育和信号转导等方面发挥重要作用。植物的CPA包括Na+/H+交换器、K+流出反向转运体和阳离子/H+交换器家族。然而，目前对蔷薇科植物CPA基因的研究还很少。本研究从5种蔷薇科植物（梨、苹果、桃、野草莓和梅）中克隆了220个CPA基因，其中53个来自P. bretschneideri。对梨CPA基因进行了系统发育、结构、共线性和基因表达分析。基因表达数据显示，有35个和37个CPA基因分别在梨果实和花粉管中表达。对一些CPA基因在非生物胁迫条件下的转录本分析表明，CPA可能在花粉管生长中起重要作用。这里提出的结果将有助于提高CPA基因家族的复杂性的理解，并将促进在未来的研究中的功能表征。
The monovalent cation proton antiporters (CPAs) play essential roles in plant nutrition, development, and signal transduction by regulating ion and pH homeostasis of the cell. The CPAs of plants include the Na+/H+exchanger, K+efflux antiporter, and cation/H+exchanger families. However, currently, little is known about the CPA genes in Rosaceae species. In this study, 220 CPA genes were identified from five Rosaceae species (Pyrus bretschneideri,Malus domestica,Prunus persica,Fragaria vesca,andPrunus mume), and 53 of which came fromP. bretschneideri. Phylogenetic, structure, collinearity, and gene expression analyses were conducted on the entire CPA genes of pear. Gene expression data showed that 35 and 37 CPA genes were expressed in pear fruit and pollen tubes, respectively. The transcript analysis of some CPA genes under abiotic stress conditions revealed that CPAs may play an important role in pollen tubes growth. The results presented here will be useful in improving understanding of the complexity of the CPA gene family and will promote functional characterization in future studies.