Biological effects of synthetic AKH in Manduca sexta and estimates of the amount of AKH in corpora cardiaca.
Biological effects of synthetic AKH in Manduca sexta and estimates of the amount of AKH in corpora cardiaca.
复制标题
Manduca sexta 中合成 AKH 的生物学效应以及贲门体中 AKH 含量的估计。
DOI：
10.1002/arch.940150206
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发表时间：
1990
影响因子：
2.2
通讯作者：
Ziegler,R
中科院分区：
文献类型：
--
作者：
Ziegler,R
使用合成的Manduca脂肪动力激素（AKH）测量幼虫糖原磷酸化酶激活和成虫脂质动员的剂量反应曲线。这两种反应是已知的激素功能在天蛾。在结扎的幼虫中，糖原磷酸化酶在0.1 pmol时达到完全激活，在0.03-0.04 pmol时达到半最大激活。成人的最大脂质动员需要10 pmol，半最大动员需要0.15至0.2 pmol。通过比较合成ManducaAKH的剂量反应曲线与腺体提取物的曲线，获得了6。还通过高效液相色谱法对心体提取物进行定量。根据这两种估计，成虫的心体每对含有10-20 pmol AKH，而幼虫的心体每对含有0.7-2 pmol AKH。
Dose‐response curves were measured with syntheticManducaadipokinetic hormone (AKH) for glycogen phosphorylase activation in larvae and for lipid mobilization in adults. Both responses are known hormonal functions inManduca sexta. In ligated larvae, full activation of glycogen phosphorylase was achieved with 0.1 pmol and half‐maximal activation with 0.03–0.04 pmol. Maximal lipid mobilization in adults required 10 pmol and half‐maximal mobilization 0.15 to 0.2 pmol, respectively.An estimate of AKH content of corpora cardiaca fromM. sextawas gained by comparing the dose‐response curves for syntheticManducaAKH with curves from gland extracts. Corpora cardiaca extracts were also quantitated by high performance liquid chromatography. According to both estimates corpora cardiaca of adults contain 10–20 pmol AKH per pair, while a pair of larval corpora cardiaca contains 0.7–2 pmol.