Local and global measures of shape dynamics
Local and global measures of shape dynamics
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DOI：
10.1088/1478-3975/8/5/055001
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发表时间：
2011-10-01
期刊：
影响因子：
2
通讯作者：
Losert, Wolfgang
中科院分区：
文献类型：
--
作者：
Driscoll, Meghan K.;Fourkas, John T.;Losert, Wolfgang
细胞的形状和运动可以提供对细胞内部运作的重要见解。我们提出了一个简单而通用的框架，它提供了细胞形状和细胞形状动力学的多个度量。对盘状盘基骨细胞迁移的分析表明，全局和局部指标突出了不同的细胞过程。例如，形状的整体测量显示有节奏的振荡，这表明收缩，而形状的局部测量显示波浪状的动态，这表明突起。从动态形状或边界运动的局部测量中，我们提取出突出和收缩的时间和位置。我们发现，突起呈锯齿状，而收缩沿边界大致保持静止。我们没有观察到任何时间关系之间的突出和收缩。我们的分析框架还提供了整个边界的度量。例如，随着细胞速度的增加，我们发现细胞的形状变得更加细长。我们还观察到，虽然伸展和收缩有相似的区域，但它们的形状不同。
The shape and motion of cells can yield significant insights into the internal operation of a cell. We present a simple, yet versatile, framework that provides multiple metrics of cell shape and cell shape dynamics. Analysis of migrating Dictyostelium discoideum cells shows that global and local metrics highlight distinct cellular processes. For example, a global measure of shape shows rhythmic oscillations suggestive of contractions, whereas a local measure of shape shows wave-like dynamics indicative of protrusions. From a local measure of dynamic shape, or boundary motion, we extract the times and locations of protrusions and retractions. We find that protrusions zigzag, while retractions remain roughly stationary along the boundary. We do not observe any temporal relationship between protrusions and retractions. Our analysis framework also provides metrics of the boundary as whole. For example, as the cell speed increases, we find that the cell shape becomes more elongated. We also observe that while extensions and retractions have similar areas, their shapes differ.