Circadian clock gene expression in the ovary: Effects of luteinizing hormone
Circadian clock gene expression in the ovary: Effects of luteinizing hormone
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DOI：
10.1095/biolreprod.106.050732
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发表时间：
2006-10-01
影响因子：
3.6
通讯作者：
Tischkau, Shelley A.
中科院分区：
文献类型：
--
作者：
Karman, Bethany N.;Tischkau, Shelley A.
在真核生物中，一种以每日或昼夜节律为尺度测量时间的分子装置几乎无处不在。时钟基因的核心组，其协调功能是需要这种计时，在中央时钟和许多外周器官中表达。我们检测了大鼠卵巢中时钟基因的表达。实时定量RT-PCR显示，核心振荡器元件（Arntl，Clock，Per 1，Per 2和Cry 1）的转录本存在于卵巢中。Arnt 1和Per 2转录本和蛋白质产物的节律性表达模式与中央振荡器的相位不同，并且彼此完全反相。在发情前期LH峰后，Arnt 1的表达显著升高。最后，hCG治疗诱导垂体切除的未成熟大鼠中Arnt 1和Per 2基因产物的周期性表达。总的来说，这些数据表明，驱动昼夜节律的转录/翻译反馈回路的核心基础存在于大鼠卵巢中。此外，该研究确定LH是卵巢生物钟基因节律的潜在调节因子。
A molecular device that measures time on a daily, or circadian, scale is a nearly ubiquitous feature of eukaryotic organisms. A core group of clock genes, whose coordinated function is required for this timekeeping, is expressed both in the central clock and within numerous peripheral organs. We examined expression of clock genes in the rat ovary. Transcripts for core oscillator elements (Arntl, Clock, Per1, Per2, and Cry1) were present in the ovary as indicated by quantitative real-time RT-PCR. Rhythmic expression patterns of Arnt1 and Per2 transcripts and protein products were out of phase with respect to the central oscillator and in complete antiphase to each other. Expression of Arnt1 was significantly elevated after the LH surge on the day of proestrus. Finally, hCG treatment induced cyclic expression of both Arnt1 and Per2 gene products in hypophy-sectomized, immature rats primed with eCG. Collectively, these data suggest that the core underpinnings of the transcriptional/translational feedback loop that drives circadian rhythmicity is present in the rat ovary. Furthermore, the study identifies LH as a potential regulator of circadian clock gene rhythms in the ovary.