Arsenic detoxification and evolution of trimethylarsine gas by a microbial arsenite S-adenosylmethionine methyltransferase
Arsenic detoxification and evolution of trimethylarsine gas by a microbial arsenite S-adenosylmethionine methyltransferase
复制标题
DOI：
10.1073/pnas.0506836103
复制
发表时间：
2006-02-14
影响因子：
11.1
通讯作者：
Rensing, C
中科院分区：
文献类型：
--
作者：
Qin, J;Rosen, BP;Rensing, C
本文描述了亚砷酸盐[As(III)]通过甲基化和随后的挥发来抵抗的机理。沼泽红假单胞菌arsM的异源表达使其对一株砷敏感的大肠杆菌产生抗性。ArsM催化从As(III)生成一些甲基化中间体，最终产物为三甲基砷。最终的结果是从培养液和细胞中都损失了砷。由于ArsM同系物在自然界中广泛存在，这种微生物介导的转化被认为对全球砷循环有重要影响。
In this article, a mechanism of arsenite [As(III)] resistance through methylation and subsequent volatization is described. Heterologous expression of arsM from Rhodopseudomonas palustris was shown to confer As(III) resistance to an arsenic-sensitive strain of Escherichia coli. ArsM catalyzes the formation of a number of methylated intermediates from As(III), with trimethylarsine as the end product. The net result is loss of arsenic, from both the medium and the cells. Because ArsM homologues are widespread in nature, this microbial-mediated transformation is proposed to have an important impact on the global arsenic cycle.