Electromagnetic Pulse Exposure Induces Overexpression of Beta Amyloid Protein in Rats
Electromagnetic Pulse Exposure Induces Overexpression of Beta Amyloid Protein in Rats
复制标题
电磁脉冲暴露诱导大鼠体内β淀粉样蛋白过度表达。
DOI：
10.1016/j.arcmed.2013.03.005
复制
发表时间：
2013-04-01
影响因子：
7.7
通讯作者：
Guo, Guo-zhen
中科院分区：
文献类型：
--
作者：
Jiang, Da-peng;Li, Jing;Guo, Guo-zhen
背景和目标。随着电磁场技术的发展和广泛应用，电磁场与阿尔茨海默病的关系越来越受到人们的关注。电磁脉冲（EMP）是一种应用广泛的电磁场。本研究旨在阐明电磁脉冲暴露是否会导致认知和记忆障碍，从而发现电磁脉冲与AD之间的可能关系。将40只健康雄性Sprague道利大鼠随机分为4组，分别于2月龄时接受100、1000和10,000脉冲EMP（场强50 kV/m，重复频率100 Hz）照射和假照射。采用Morris水迷宫（MWM）每月一次检测大鼠认知和记忆能力的变化。以超氧化物歧化酶（SOD）活性和谷胱甘肽（GSH）含量为氧化应激指标。同时检测了阿尔茨海默病相关蛋白的表达。电磁脉冲暴露后，与假暴露组相比，可引起明显的认知和记忆障碍（p < 0.05）。氧化指标测定显示，与假手术组相比，暴露组SOD活性和GSH含量降低。免疫组织化学染色显示EMP暴露组与假手术组相比，β淀粉样蛋白（A β）增加。Western blot实验显示EMP暴露组A β寡聚体和β淀粉样蛋白前体（APP）表达增加。微管相关蛋白1轻链3-II（LC 3-II）表达增加。本研究结果表明，电磁脉冲暴露可导致大鼠认知和记忆功能的长期损害，从而出现AD样症状。这可能是通过增强氧化应激诱导的，并且与自噬功能障碍有关。(C)2013年IMSS。爱思唯尔公司出版
Background and Aims. With the developing and widely used electromagnetic field (EMF) technology, more and more studies are focusing on the relationship between EMF and Alzheimer's disease (AD). Electromagnetic pulse (EMP) is one type of widely used EMF. This study aimed to clarify whether EMP exposure could induce cognitive and memory impairment, thus finding a possible relationship between EMP and AD.Methods. Forty healthy male Sprague Dawley rats were randomly divided into four groups Animals, respectively, received 100, 1000, and 10,000 pulses EMP (field strength 50 kV/m, repetition rate 100 Hz) exposure and sham exposure when 2 months old. Monthly Morris water maze (MWM) was used to test the changes of cognitive and memory ability. Superoxide dismutase (SOD) activity and glutathione (GSH) content were used as oxidative stress indexes. Expressions of some types of Alzheimer's disease-related proteins were also detected.Results. After exposure, EMP exposure caused clear cognitive and memory impairment compared with sham exposure group (p < 0.05). Determination of oxidation indexes showed decreased SOD activity and GSH content in exposure groups compared with sham group. Immunohistochemical (IHC) staining showed increased beta amyloid protein (A beta) in EMP exposure groups compared with sham group. Western blot experiments showed increased expressions of A beta oligomer and beta amyloid protein precursor (APP) in EMP exposure groups. Increased expression of microtubule-associated protein 1 light chain 3-II (LC3-II) was also found.Conclusions. The present results showed that EMP exposure can cause long-term impairment in impaired cognition and memory of rats, resulting in AD-like symptoms. This may be induced by enhancing oxidative stress and is related to autophagy dysfunction. (C) 2013 IMSS. Published by Elsevier Inc.