RAPID SAMPLING AND DETERMINATION OF EXTRACELLULAR DOPAMINE INVIVO
RAPID SAMPLING AND DETERMINATION OF EXTRACELLULAR DOPAMINE INVIVO
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DOI：
10.1021/ac00132a007
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发表时间：
1987-03-01
影响因子：
7.4
通讯作者：
JUSTICE, JB
中科院分区：
文献类型：
--
作者：
CHURCH, WH;JUSTICE, JB
利用微透析技术对大鼠脑细胞外液中存在的神经递质多巴胺进行了快速采样和分析。0.5 -.mu集中。采用210 nL/min的灌注流速获得L样品。用小口径（1.0 mm）液相色谱柱和电化学检测进行5分钟分析。多巴胺的滞留时间为3分钟。分析的时间尺度适合于研究急性药理学和行为学操作。色谱系统的评价依据是检出限和对采样介质浓度变化的动态响应。在体外和体内对该系统的性能进行了表征。该系统可在0.5-.mu中常规测量20 nM多巴胺。L样本。
Rapid sampling and analysis of the neurotransmitter dopamine present in the extracellular fluid of rat brain have been achieved by use of microdialysis. Concentrated 0.5-.mu.L samples are obtained by using a perfusion flow rate of 210 nL/min. A 5-min analysis is performed with a smallbore (1.0 mm i.d.) liquid chromatographic column and electrochemical detection. The retention time for dopamine is 3 min. The time scale of the analysis is appropriate for the study of acute pharmacological and behavioral manipulations. The chromatographic system is evaluated on the basis of detection limit and dynamic response to concentration changes in the sampled medium. The performance of the system is characterized in vitro and in vivo. The system can routinely measure 20 nM dopamine in 0.5-.mu. L samples.