A role for ion homeostasis in yeast ionic liquid tolerance.
A role for ion homeostasis in yeast ionic liquid tolerance.
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DOI：
10.17912/micropub.biology.000718
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发表时间：
2023
影响因子：
--
通讯作者：
Sato, Trey K
中科院分区：
文献类型：
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作者：
Liu, Lisa;Ansari, Rahim U;Vang-Smith, Maikayeng;Hittinger, Chris Todd;Sato, Trey K
正在开发的模式酵母酿酒酵母（Saccharomyces cerevisiae）作为生物催化剂，用于将可再生木质纤维素生物质转化为生物燃料。离子液体1-乙基-3-甲基咪唑鎓氯化物（EMIMCl）溶解木质纤维素用于解构成可发酵糖，但其抑制酵母发酵。EMIMCl耐受性由外排泵Sge 1 p和未表征的蛋白Ilt 1 p介导。通过遗传学研究，我们发现，通过编码Trk 1 p钾转运蛋白及其蛋白激酶调节剂Sat 4p和Hal 5 p的基因突变破坏离子稳态，导致EMIMCl敏感性。这些结果表明，离子稳态的维持对于EMMCl的耐受性是重要的。
The model yeast Saccharomyces cerevisiae is being developed as a biocatalyst for the conversion of renewable lignocellulosic biomass into biofuels. The ionic liquid 1-ethyl-3-methylimidazolium chloride (EMIMCl) solubilizes lignocellulose for deconstruction into fermentable sugars, but it inhibits yeast fermentation. EMIMCl tolerance is mediated by the efflux pump Sge1p and uncharacterized protein Ilt1p. Through genetic investigation, we found that disruption of ion homeostasis through mutations in genes encoding the Trk1p potassium transporter and its protein kinase regulators, Sat4p and Hal5p, causes EMIMCl sensitivity. These results suggest that maintenance of ion homeostasis is important for tolerance to EMIMCl.