Mobilization and fusion of a non-recycling pool of synaptic vesicles under conditions of endocytic blockade
Mobilization and fusion of a non-recycling pool of synaptic vesicles under conditions of endocytic blockade
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DOI：
10.1016/j.neuropharm.2004.07.026
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发表时间：
2004-10-01
影响因子：
4.7
通讯作者：
Davis, GW
中科院分区：
文献类型：
--
作者：
Poskanzer, KE;Davis, GW
在脊椎动物的中枢突触中，已经证明存在突触囊泡（SV）的静息池，其通常不参与SV的释放和再循环。目前尚不清楚静止池中的SV是否能够动员和融合。在这里，我们结合联合收割机现场成像SV外吞和内吞使用pH敏感的GFP（突触pHluorins）与药理学和遗传操纵的SV周期在果蝇NMJ。我们证明，一个休息池的SV存在于这个突触，包括总SV池的30-41%。在内吞阻断的条件下，使用温度敏感的发动蛋白突变，可以动员和释放SV的静息池。我们提出了一个模型的存在下，不需要分子规格的一个亚群的SV的静止池。(C)2004 Elsevier Ltd.保留所有权利。
At vertebrate central synapses, it has been demonstrated that a resting pool of synaptic vesicles (SVs) exists that normally does not participate in SV release and recycling. It remains unclear whether SVs within the resting pool are capable of mobilization and fusion. Here, we combine live imaging of SV exo- and endocytosis using pH-sensitive GFP (synapto-pHluorins) with pharmacological and genetic manipulations of the SV cycle at the Drosophila NMJ. We demonstrate that a resting pool of SVs exists at this synapse that encompasses 30-41% of the total SV pool. Under conditions of endocytic blockade, using a temperature-sensitive dynamin mutation, the resting pool of SVs can be mobilized and released. We present a model for the presence of a resting pool of SVs that does not require molecular specification of a subpopulation of SVs. (C) 2004 Elsevier Ltd. All rights reserved.