TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis.
TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis.
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DOI：
10.7554/elife.66709
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发表时间：
2021-09-27
期刊：
影响因子：
7.7
通讯作者：
Gong LW
中科院分区：
文献类型：
--
作者：
Jiang ZJ;Li W;Yao LH;Saed B;Rao Y;Grewe BS;McGinley A;Varga K;Alford S;Hu YS;Gong LW
瞬时受体电位美拉他汀7 （TRPM7）在许多组织和细胞中参与多种生理和病理过程。TRPM7广泛分布于神经系统，参与动物行为和缺血引起的神经元死亡。然而，TRPM7在中枢神经系统（CNS）神经元中的生理作用尚不清楚。在这里，我们在TRPM7敲除（KO）小鼠的神经内分泌细胞和神经元中发现了内吞缺陷，表明TRPM7在突触囊泡内吞作用中的作用。我们的实验进一步明确了TRPM7作为离子通道在突触囊泡内吞作用中的重要性。Ca2+成像检测到TRPM7 KO神经元突触前Ca2+动力学缺陷，提示通过TRPM7的Ca2+内流在突触囊泡内吞中的重要性。此外，TRPM7 KO小鼠的兴奋性和抑制性突触传递均增强了短期抑制。综上所述，我们的数据表明，通过TRPM7的Ca2+内流可能通过调节神经元突触囊泡内吞作用对突触强度的短期可塑性至关重要。
Transient receptor potential melastatin 7 (TRPM7) contributes to a variety of physiological and pathological processes in many tissues and cells. With a widespread distribution in the nervous system, TRPM7 is involved in animal behaviors and neuronal death induced by ischemia. However, the physiological role of TRPM7 in central nervous system (CNS) neuron remains unclear. Here, we identify endocytic defects in neuroendocrine cells and neurons from TRPM7 knockout (KO) mice, indicating a role of TRPM7 in synaptic vesicle endocytosis. Our experiments further pinpoint the importance of TRPM7 as an ion channel in synaptic vesicle endocytosis. Ca2+ imaging detects a defect in presynaptic Ca2+ dynamics in TRPM7 KO neuron, suggesting an importance of Ca2+ influx via TRPM7 in synaptic vesicle endocytosis. Moreover, the short-term depression is enhanced in both excitatory and inhibitory synaptic transmissions from TRPM7 KO mice. Taken together, our data suggests that Ca2+ influx via TRPM7 may be critical for short-term plasticity of synaptic strength by regulating synaptic vesicle endocytosis in neurons.
影响因子：
7.8
作者：
Ceccarelli, B;Hurlbut, W P
通讯作者：
Hurlbut, W P