A 39-KDA PROTEIN ON ACTIVATED HELPER T-CELLS BINDS CD40 AND TRANSDUCES THE SIGNAL FOR COGNATE ACTIVATION OF B-CELLS
A 39-KDA PROTEIN ON ACTIVATED HELPER T-CELLS BINDS CD40 AND TRANSDUCES THE SIGNAL FOR COGNATE ACTIVATION OF B-CELLS
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DOI：
10.1073/pnas.89.14.6550
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发表时间：
1992-07-15
影响因子：
11.1
通讯作者：
ARUFFO, A
中科院分区：
文献类型：
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作者：
NOELLE, RJ;ROY, M;ARUFFO, A
CD40是一种B细胞表面分子，已被证明与单抗连接后可诱导B细胞生长。这份报告显示，CD40的触发对于辅助性T细胞(T(H))激活静息B细胞是必不可少的。CD40与人免疫球蛋白的融合蛋白CD40-Ig可抑制活化的T(H)1和T(H)2诱导的B细胞周期进入、增殖和分化。CD40的配体是一种39 kDa的膜蛋白，选择性表达于活化的T(H)上。针对39 kDa蛋白的单抗可抑制CD40-Ig结合，也可抑制T(H)对B细胞的激活作用。这些数据表明，活化的T(H)上表达的39 kDa膜蛋白是CD40的结合蛋白，其功能是转导T(H)依赖的B细胞激活的信号。
CD40 is a B-cell surface molecule that has been shown to induce B-cell growth upon ligation with monoclonal antibodies. This report shows that triggering via CD40 is essential for the activation of resting B cells by helper T cells (T(h)). A soluble fusion protein of CD40 and human immunoglobulin, CD40-Ig, inhibited the induction of B-cell cycle entry, proliferation, and differentiation by activated T(h)1 and T(h)2. The ligand for CD40 was identified as a 39-kDa membrane protein that was selectively expressed on activated T(h). A monoclonal antibody specific for the 39-kDa protein inhibited CD40-Ig binding and also inhibited the activation of B cells by T(h). These data indicate that the 39-kDa membrane protein expressed on activated T(h) is a binding protein for CD40 and functions to transduce the signal for T(h)-dependent B-cell activation.