Perikaryal cell labeling in the subthalamic nucleus following the injection of 3h-γ-aminobutyric acid into the pallidal complex: An autoradiographic study in cat
Perikaryal cell labeling in the subthalamic nucleus following the injection of 3h-γ-aminobutyric acid into the pallidal complex: An autoradiographic study in cat
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将 3h-γ-氨基丁酸注射到苍白球复合体后，丘脑底核中的核周细胞标记：猫放射自显影研究
DOI：
10.1016/0306-4522(82)90096-3
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发表时间：
1982
期刊：
影响因子：
3.3
通讯作者：
M. Cuénod
中科院分区：
文献类型：
--
作者：
H. Nauta;M. Cuénod
虽然丘脑底核被认为对纹状体输出细胞有重要影响，但有关递质的信息却很少。在一系列的放射自显影实验中，各种不同的放射性标记的假定递质物质分别注入猫的苍白球复合体中，注意到~3H-γ-氨基丁酸注射持续地导致下丘脑核的核周标记。~H-γ-氨基丁酸注射在苍白球外核段的外侧部分导致标记细胞位于丘脑下核的外侧，而~H-氨基丁酸注射在苍白内侧核(内侧未见核)导致细胞更内侧地标记在丘脑下核。在全身性氨基氧乙酸预处理的猫苍白球外段注射~3H-γ-氨基丁酸后，在外侧壳核也观察到周围细胞的标记。除纹状体和丘脑底核外，不能使其他细胞群标记~3H-γ-氨基丁酸。此外，在苍白球复合体注射~3H-d-天冬氨酸或~3H-5-羟色胺后，未见丘脑底核有核周细胞标记。结果表明，~H-γ-氨基丁酸，而不是天冬氨酸或5-羟色胺，经历了高亲和力摄取和逆行运输的下丘脑-苍白球神经元。考虑到所讨论的许多保留意见，该观察表明，~H-γ-氨基丁酸可能是下丘脑-苍白球通路中的一种递质。
Although the subthalamic nucleus is thought to exert a major influence on the corpus striatum output cells, there is little information available on the transmitter or transmitters involved.In a series of autoradiographic experiments in which various different radiolabeled putative transmitter substances were injected separately into the pallidal complex of cats, it was noted that3H-gamma-aminobutyric acid injection consistently resulted in perikaryal labeling in the subthalamic nucleus.3H-γ-aminobutyric acid injection in the lateral part of the external pallidal segment resulted in labeled cells situated laterally in the subthalamic nucleus, while3H-aminobutyric acid injection in the internal pallidal segment (entopeduncular nucleus) resulted in cell labeling more medially in the subthalamic nucleus. Perikaryal cell labeling was also noted in the lateral putamen following3H-γ-aminobutyric acid injection in the external pallidal segment in cats pretreated with systemic amino-oxyacetic acid. No cell groups other than the striatum and subthalamic nucleus could be made to label with3H-γ-aminobutyric acid. Furthermore, no perikaryal cell labeling in the subthalamic nucleus was seen to follow injections of3H-d-aspartate or3H-serotonin into the pallidal complex. The findings suggest that3H-γ-aminobutyric acid, but not aspartate or serotonin, undergoes high affinity uptake and retrograde transport by subthalamo-pallidal neurons. Bearing in mind the many reservations discussed, the observation implies that3H-γ-aminobutyric acid may be a transmitter in the subthalamo-pallidal pathway.