The role of cell-released microvesicles in the intercellular transfer of magnetic nanoparticles in the monocyte/macrophage system
The role of cell-released microvesicles in the intercellular transfer of magnetic nanoparticles in the monocyte/macrophage system
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DOI：
10.1016/j.biomaterials.2010.05.062
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发表时间：
2010-09-01
期刊：
影响因子：
14
通讯作者：
Gazeau, Florence
中科院分区：
文献类型：
--
作者：
Luciani, Nathalie;Wilhelm, Claire;Gazeau, Florence
单核巨噬细胞系统是纳米材料注射的第一靶点。单核细胞/巨噬细胞是体内磁性纳米颗粒运输和清除的主要参与者。然而，纳米磁性标签的命运是一个持续的问题。在这里，我们证明了单核细胞/巨噬细胞系统显示出复杂的动态行为，相对于铁氧化物纳米颗粒的摄取。一旦被单核细胞或巨噬细胞内化，磁性纳米颗粒可以在应激或激活时被细胞释放。我们确定了由活化或凋亡细胞脱落的膜囊泡作为运输磁性纳米材料的载体。这些囊泡被幼稚巨噬细胞吸收并触发磁性标记的细胞间转移。它们还导致在磁性标记的单核细胞分化成巨噬细胞期间磁性标记的重新分布。由细胞释放的囊泡介导的磁性标记的细胞间转移使生物体中的纳米颗粒结果的图片及其作为MRI细胞示踪剂的用途复杂化。(C)2010爱思唯尔有限公司版权所有。
Mononuclear phagocyte system is the first target for injected nanomaterials. Monocytes/macrophages are the central actors for trafficking and clearance of magnetic nanoparticles in vivo. However the fate of nanosized magnetic label is an ongoing issue. Here we demonstrate that the monocyte/macrophage system shows a complex dynamic behavior with respect to iron-oxide nanoparticles uptake. Once internalized by monocytes or macrophages, magnetic nanoparticles can be released by cells upon stress or activation. We identified membrane vesicles shed by activated or apoptotic cells as the carriers transporting magnetic nanomaterials. These vesicles are taken up by naive macrophages and trigger an intercellular transfer of the magnetic label. They also lead to the redistribution of magnetic label during differentiation of magnetically-labeled monocytes into macrophages. Intercellular transfer of magnetic label, mediated by cell-released vesicles, complicates the picture of nanoparticles outcome in the organism and their use as MRI cell tracers. (C) 2010 Elsevier Ltd. All rights reserved.