Cryopreservation of ovine embryos:: Slow freezing and vitrification
Cryopreservation of ovine embryos:: Slow freezing and vitrification
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DOI：
10.1016/s0093-691x(98)00052-1
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发表时间：
1998-04-01
期刊：
影响因子：
2.8
通讯作者：
Matkovic, M
中科院分区：
文献类型：
--
作者：
Martínez, AG;Matkovic, M
在体外（体外培养后的存活率）和体内（田间条件下转移后的妊娠率和产羔率）评价了绵羊胚胎冷冻保存的不同方法。在前2个实验中，评估了缓慢冷冻条件。当甘油和乙二醇进行比较时，没有发现总妊娠率的差异（40.2%对51.3%），但在桑椹胚中乙二醇比甘油获得更好的结果（29.7%对59.4%，P
Different methods for the cryopreservation of ovine embryos were evaluated in vitro (survival upon culture in vitro) and in vivo (pregnancy and lambing rates after transfer in field conditions). In the first 2 experiments, slow freezing conditions were evaluated. When glycerol and ethylene glycol were compared, no differences in the overall pregnancy rate were found (40.2 vs 51.3%), but better results were obtained with ethylene glycol than with glycerol in morulae (29.7 vs 59.4%, P