Interpreting simple STR mixtures using allele peak areas
Interpreting simple STR mixtures using allele peak areas
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DOI：
10.1016/s0379-0738(97)00174-6
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发表时间：
1998-01-09
影响因子：
2.2
通讯作者：
Buckleton, J
中科院分区：
文献类型：
--
作者：
Gill, P;Sparkes, R;Buckleton, J
虽然现有的统计模型可以解释混合物定性的基础上存在的等位基因，使用自动测序仪打开了机会，考虑到定量方面体现的峰面积。理解仅由两个供体组成的简单混合物的一个步骤是估计混合比。当给定基因座上存在明显的四个等位基因混合物时，这相对容易做到。然而，如果混合物由三个或更少的等位基因组成，则该过程并不简单。我们证明，混合物的估计是一致的，在一个多重系统中的所有位点。一旦混合比已知，则可以使用简单的电子表格分析来估计任何给定等位基因组合的预期峰面积。(C)1998爱思唯尔科学爱尔兰有限公司
Although existing statistical models can interpret mixtures qualitatively based upon the alleles present, the use of automated sequencers opens the opportunity to take account of quantitative aspects embodied by the peak area. One step in understanding simple mixtures consisting of just two donors is to estimate the mixture ratio. This is relatively easy to do when four-allele mixtures are evident at a given locus. However, if the mixture consists of three or fewer alleles, the process it is not straightforward. We demonstrate that mixture estimates are consistent across all loci in a multiplex system. Once the mixture ratio is known, then the expected peak areas for any given combination of alleles can be estimated using a simple spreadsheet analysis. (C) 1998 Elsevier Science Ireland Ltd.