SYCE2 is required for synaptonemal complex assembly, double strand break repair, and homologous recombination.
SYCE2 is required for synaptonemal complex assembly, double strand break repair, and homologous recombination.
复制标题
SYCE2是突发型复合物组件，双链断裂修复和同源重组所必需的。
DOI：
10.1083/jcb.200610027
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发表时间：
2007-03-12
影响因子：
7.8
通讯作者：
Cooke, Howard J
中科院分区：
文献类型：
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作者：
Bolcun-Filas, Ewelina;Costa, Yael;Speed, Robert;Taggart, Mary;Benavente, Ricardo;De Rooij, Dirk G;Cooke, Howard J
联会是配对的染色体同源物在减数分裂中在交换之前紧密结合的过程。在联会复合体（SC）中，每个同源物的轴向元件通过SYCP 1分子连接到中心元件，中心元件包含蛋白质SYCE 1和-2。我们已经衍生出缺乏SYCE 2蛋白的小鼠，产生雄性和雌性小鼠，其中减数分裂染色体对齐，轴形成，但不突触。性染色体是未排列的，不形成性体。此外，DNA断裂和修复的标记保留在轴上，并且交换受损，最终导致雄性和雌性都不能产生配子。我们表明，SC的形成可以启动SYCE1/SYCP1本地化的网站，但这些点的起始不能在SYCE2的情况下延长。因此，SC组件依赖于SYCP 1、SYCE 1和SYCE 2。我们提供了一个基于蛋白质-蛋白质相互作用的模型来解释这一点。
Synapsis is the process by which paired chromosome homologues closely associate in meiosis before crossover. In the synaptonemal complex (SC), axial elements of each homologue connect through molecules of SYCP1 to the central element, which contains the proteins SYCE1 and -2. We have derived mice lacking SYCE2 protein, producing males and females in which meiotic chromosomes align and axes form but do not synapse. Sex chromosomes are unaligned, not forming a sex body. Additionally, markers of DNA breakage and repair are retained on the axes, and crossover is impaired, culminating in both males and females failing to produce gametes. We show that SC formation can initiate at sites of SYCE1/SYCP1 localization but that these points of initiation cannot be extended in the absence of SYCE2. SC assembly is thus dependent on SYCP1, SYCE1, and SYCE2. We provide a model to explain this based on protein–protein interactions.