A comparative study of endothelial cell markers expressed in chronically inflamed human tissues: MECA-79, Duffy antigen receptor for chemokines, von Willebrand factor, CD31, CD34, CD105 and CD146
A comparative study of endothelial cell markers expressed in chronically inflamed human tissues: MECA-79, Duffy antigen receptor for chemokines, von Willebrand factor, CD31, CD34, CD105 and CD146
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DOI：
10.1002/path.1788
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发表时间：
2005-07-01
影响因子：
7.3
通讯作者：
Girard, JP
中科院分区：
文献类型：
--
作者：
Middleton, J;Americh, L;Girard, JP
内皮细胞在慢性炎症中起着核心作用：例如，它们表达粘附分子并呈递趋化因子，导致白细胞向组织中的募集增强。已经报道了许多内皮细胞的标志物，但缺乏关于其特异性的比较数据。本研究比较了克罗恩病患者类风湿滑膜和结肠中7种内皮细胞标志物的特异性。这些标志物是：硫酸化表位MECA-79、Duffy抗原趋化因子受体（DARC）、von Willebrand因子、CD 31（PECAM-1）、CD 34、CD 105（内皮糖蛋白）和CD 146。NIECA-79、DARC和vonWillebrand因子在内皮细胞中有特异性分布。识别白细胞粘附受体L-选择素（CD 62 L）的硫酸化配体的NIECA-79对高度炎症组织中的小静脉子集具有选择性，并且存在于类风湿性关节炎滑膜中，但不存在于对照骨关节炎滑膜中。DARC对蠕虫也有特异性，但比NIECA-79分布更广，并且存在于类风湿和对照滑膜中。其他标记物均标记血管、小动脉和毛细血管中的内皮细胞。然而，它们也定位于其他细胞类型。例如，CD 34染色成纤维细胞，CD 146由血管壁的周细胞和平滑肌细胞表达，CD 31和CD 105标记广泛的细胞类型。版权所有（c）2005大不列颠和爱尔兰病理学会。出版社：John Wiley & Sons，Ltd
Endothelial cells play a central role in chronic inflammation: for example, they express adhesion molecules and present chemokines leading to enhanced leukocyte recruitment into tissues. Numerous markers of endothelial cells have been reported but there has been a lack of comparative data on their specificity. The present study compared the specificity of seven endothelial cell markers in the rheumatoid synovium and the colon of patients with Crohn's disease. These markers were: the sulphated epitope MECA-79, the Duffy antigen receptor for chemokines (DARC), von Willebrand factor, CD31 (PECAM-1), CD34, CD105 (endoglin) and CD146. NIECA-79, DARC and von Willebrand factor showed a specific endothelial cell distribution. NIECA-79, which recognizes sulphated ligands for leukocyte adhesion receptor L-selectin (CD62L), was selective for a subset of venules in highly inflamed tissue and was present in rheumatoid but not control osteoarthritic synovia. DARC was also specific for vermles but had a more widespread distribution than NIECA-79, and was present in rheumatoid and control synovia. The other markers all labelled endothelial cells in vermles, arterioles and capillaries. However, they also localized to other cell types. For example, CD34 stained fibroblasts, CD146 was expressed by the pericytes and smooth muscle cells of vessel walls and CD31 and CD105 labelled a broad range of cell types. Copyright (c) 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.