COMPARISON OF NEUTRALIZATION OF BPV-1 INFECTION OF C127 CELLS AND BOVINE FETAL SKIN XENOGRAFTS
COMPARISON OF NEUTRALIZATION OF BPV-1 INFECTION OF C127 CELLS AND BOVINE FETAL SKIN XENOGRAFTS
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DOI：
10.1002/ijc.2910490224
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发表时间：
1991-09-09
影响因子：
6.4
通讯作者：
JENSEN, AB
中科院分区：
文献类型：
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作者：
GHIM, S;CHRISTENSEN, ND;JENSEN, AB
BPV-1诱导小鼠C127细胞和牛胎儿皮肤异种移植物中纤维乳头状瘤的病灶形成。 在这项研究中，我们比较了特异性中和BPV-1在两种检测系统中，使用血清和单克隆抗体（MAbs）选择定义中和表位。 接种完整BPV-1或BPV-2病毒粒子的兔和牛血清可中和BPV-1在C127细胞和异种移植物中的感染性。 与完整BPV-1颗粒、用变性BPV-1颗粒免疫的兔血清和用重组L1融合蛋白免疫的小牛血清反应的所选人血清和鼠单克隆抗体在任一系统中均不能中和BPV-1感染。 由此得出结论，超免疫血清对BPV-1感染C127细胞和牛胎皮肤异种移植物的中和作用对于两种检测系统具有特异性和一致性，并且涉及构象BPV-1衣壳表位。
BPV-1 induces focus formation in murine C127 cells and fibropapillomas in bovine fetal skin xenografts. In this study, we compared the specificity of neutralization of BPV-1 in both assay systems, using sera and monoclonal antibodies (MAbs) selected to define neutralizing epitopes. Sera from rabbits and cattle, inoculated with intact BPV-1 or BPV-2 virions, neutralize BPV-1 infectivity in both C127 cells and xenografts. Selected human sera and murine MAbs that react with intact BPV-1 particles, serum of a rabbit immunized with denatured BPV-1 particles, and sera from calves vaccinated with a recombinant L1 fusion protein did not neutralize BPV-1 infection in either system. It was concluded that neutralization of BPV-1 infection of C127 cells and bovine fetal skin xenografts by hyperimmune sera is specific and concordant for both assay systems, and involves conformational BPV-1 capsid epitopes.